Bioremediation of chromate: thermodynamic analysis of the effects of Cr(VI) on sulfate-reducing bacteria

被引:80
作者
Chardin, B
Dolla, A
Chaspoul, F
Fardeau, ML
Gallice, P
Bruschi, M
机构
[1] Inst Biol Struct & Microbiol, CNRS, Lab Bioenerget & Ingn Prot, F-13009 Marseille, France
[2] Fac Pharm Marseille, Lab Chim Gen & Prevent Risques & Nuidances Techno, F-13385 Marseille 05, France
[3] Univ Aix Marseille 1, Microbiol Lab, IRD, CESB,ESIL, F-13288 Marseille 9, France
关键词
D O I
10.1007/s00253-002-1091-8
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Developing new bioremediation processes for soils and effluents polluted by Cr(VI) requires the selection of the most efficient and the most heavy-metal-resistant bacteria. The effects of Cr(VI) on bioenergetic metabolism in two sulfate-reducing bacteria (SRB), Desulfovibrio vulgaris Hildenborough and Desulfomicrobium norvegicum, were monitored using isothermal microcalorimetry. The complete reduction of Cr(VI) to Cr(III) was studied by spectrophotometry and by speciation using a combination of high-performance liquid chromatography and inductively coupled plasma-mass spectrometry. Results revealed that Cr(VI) induces an inhibition of growth with concomitant production of energy, which can be compared to the reaction of the bacteria to a stress such as oxidative stress. Moreover, the sensitivity of bacteria towards this metal is as a characteristic of the strain, which leads to differences in the kinetics of Cr(VI) reduction. The study by microcalorimetry of heavy metal effects on SRB bioenergetic metabolism thus appears an appropriate tool to identify better strains to be used for industrial bioremediation process development.
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页码:352 / 360
页数:9
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