Differential transmission of actin motion within focal adhesions

被引：406

作者：

Hu, Ke ^{[1
]}

Ji, Lin ^{[1
]}

Applegate, Kathryn T. ^{[1
]}

Danuser, Gaudenz ^{[1
]}

Waterman-Storer, Clare M. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

SCIENCE | 2007年 / 315卷 / 5808期

关键词：

D O I：

10.1126/science.1135085

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Cell migration requires the transmission of motion generated in the actin cytoskeleton to the extracellular environment through a complex assembly of proteins in focal adhesions. We developed correlational fluorescent speckle microscopy to measure the coupling of focal-adhesion proteins to actin filaments. Different classes of focal-adhesion structural and regulatory molecules exhibited varying degrees of correlated motions with actin filaments, indicating hierarchical transmission of actin motion through focal adhesions. Interactions between vinculin, talin, and actin filaments appear to constitute a slippage interface between the cytoskeleton and integrins, generating a molecular clutch that is regulated during the morphodynamic transitions of cell migration.

引用

页码：111 / 115

页数：5

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