Cytotoxic effect and apoptosis induction by silver nanoparticles in HeLa cells

被引：225

作者：

Miura, Nobuhiko ^{[1
]}

Shinohara, Yasushi ^{[2
]}

机构：

[1] Natl Inst Occupat Safety & Hlth JNIOSH, Div Hlth Effects Res, Tama Ku, Kawasaki, Kanagawa 2148585, Japan

[2] Natl Inst Occupat Safety & Hlth JNIOSH, Div Work Environm Res, Tama Ku, Kawasaki, Kanagawa 2148585, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 390卷 / 03期

关键词：

Silver nanoparticles; Silver nitrate; Apoptosis; Metallothionein; Heme oxygenase; NANOSILVER; METALLOTHIONEIN; TOXICITY; PARTICLES; FABRICS; MODE; IONS;

D O I：

10.1016/j.bbrc.2009.10.039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nanosilver has well-known antibacterial properties, and is widely used in daily life as various medical and general products. In comparison with silver ion, there is serious lacking of information concerning the biological effects of nanoAg. in this study, we observed the cytotoxic effect of nanoAg in HeLa cells. The nanoAg-induced cytotoxicity was lower than that of AgNO(3), used as a silver ion source. Apoptosis evaluated by flowcytometric analysis was associated with this cell death. Further, the expressions of ho-1 and mt-2A, well-known oxidative stress-related genes, were Lip-regulated by nanoAg treatment. Our results showed that nanoAg possesses the potential for cytotoxicity, therefore, in the case of exposure at high concentrations, we should consider to protect from nanoAg-induced toxicity. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：733 / 737

页数：5

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