Transgenic expression of a gene encoding a synthetic antimicrobial peptide results in inhibition of fungal growth in vitro and in planta

被引:97
作者
Cary, JW
Rajasekaran, K
Jaynes, JM
Cleveland, TE
机构
[1] USDA ARS, So Reg Res Ctr, Food & Feed Safety Res Unit, New Orleans, LA 70124 USA
[2] Demegen Inc, Pittsburgh, PA 15221 USA
关键词
Aspergillus flavus; Colletotrichum destructivum; disease resistance; Nicotiana tabacum; synthetic peptide; transgenic; Verticillium dahliae;
D O I
10.1016/S0168-9452(00)00189-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transgenic tobacco plants producing the synthetic antimicrobial peptide D4E1, encoded by a gene under the control of an enhanced cauliflower mosaic virus 35S RNA promoter, were obtained by Agrobacterium-mediated transformation. Successful transformation was demonstrated by PCR and Southern hybridization analysis of tobacco DNAs. Expression of the synthetic D4E1 gene was shown by RT-PCR of tobacco mRNA. Crude protein extracts from leaf tissue of transformed plants significantly reduced the number of fungal colonies arising from germinating conidia of Aspergillus flavus and Verticillium dahliae by up to 75 and 99%, respectively, compared to extracts from plants transformed with pBI121. Compared to negative controls, tobacco plants expressing the D4E1 gene showed greater levels of disease resistance in planta to the fungal pathogen, Colletotrichum destructivum, which causes anthracnose. (C) 2000 Published by Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:171 / 181
页数:11
相关论文
共 37 条
[1]   Enhanced resistance to bacterial infection by Erwinia carotovora subsp atroseptica in transgenic potato plants expressing the attacin or the cecropin SB-37 genes [J].
Arce, P ;
Moreno, M ;
Gutierrez, M ;
Gebauer, M ;
Dell'Orto, P ;
Torres, H ;
Acuña, I ;
Oliger, P ;
Venegas, A ;
Jordana, X ;
Kalazich, J ;
Holuigue, L .
AMERICAN JOURNAL OF POTATO RESEARCH, 1999, 76 (03) :169-177
[2]   Resistance to Botrytis cinerea in scented geranium transformed with a gene encoding the antimicrobial protein Ace-AMP1 [J].
Bi, YM ;
Cammue, BPA ;
Goodwin, PH ;
KrishnaRaj, S ;
Saxena, PK .
PLANT CELL REPORTS, 1999, 18 (10) :835-840
[3]  
BOWLES DJ, 1990, ANNU REV BIOCHEM, V59, P873, DOI 10.1146/annurev.bi.59.070190.004301
[4]  
Broekaert WF, 1997, CRIT REV PLANT SCI, V16, P297, DOI 10.1080/713608148
[5]  
BUGOS RC, 1995, BIOTECHNIQUES, V19, P734
[6]   Cecropin A-derived peptides are potent inhibitors of fungal plant pathogens [J].
Cavallarin, L ;
Andreu, D ;
Segundo, BS .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (03) :218-227
[7]   CHANNEL-FORMING PROPERTIES OF CECROPINS AND RELATED MODEL COMPOUNDS INCORPORATED INTO PLANAR LIPID-MEMBRANES [J].
CHRISTENSEN, B ;
FINK, J ;
MERRIFIELD, RB ;
MAUZERALL, D .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5072-5076
[8]   PLANT GENE-EXPRESSION IN RESPONSE TO PATHOGENS [J].
COLLINGE, DB ;
SLUSARENKO, AJ .
PLANT MOLECULAR BIOLOGY, 1987, 9 (04) :389-410
[9]   Antifungal peptides: potential candidates for the treatment of fungal infections [J].
De Lucca, AJ .
EXPERT OPINION ON INVESTIGATIONAL DRUGS, 2000, 9 (02) :273-299
[10]   Integrons and gene cassettes: a genetic construction kit for bacteria [J].
Bennett, PM .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 1999, 43 (01) :1-4