Generation of small insert genomic FISH probes with high signal intensity suitable for deletion mapping

被引：1

作者：

Dreyling, MH

Olopade, OI

Bohlander, SK

机构：

[1] UNIV CHICAGO,DEPT MED,HEMATOL ONCOL SECT,CHICAGO,IL 60637

[2] UNIV GOTTINGEN,INST HUMANGENET,D-3400 GOTTINGEN,GERMANY

来源：

CYTOGENETICS AND CELL GENETICS | 1997年 / 76卷 / 3-4期

关键词：

YEAST ARTIFICIAL CHROMOSOMES;

D O I：

10.1159/000134549

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have developed a method to generate FISH probes from small (2-4 kb) nonrepetitive genomic restriction fragments. The probes showed a high hybridization efficiency of up to 90 % in metaphase cells from normal peripheral blood. With the use of these probes, homozygous as well as hemizygous 9p deletions were reliably identified in nine leukemia-derived cell lines.

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页码：202 / 205

页数：4

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