Interaction of Shigella flexneri IpaC with model membranes correlates with effects on cultured cells

被引：22

作者：

Tran, N

Serfis, AB

Osiecki, JC

Picking, WL

Coye, L

Davis, R

Picking, WD

机构：

[1] Univ Kansas, Dept Mol Biosci, Lawrence, KS 66045 USA

[2] St Louis Univ, Dept Chem, St Louis, MO 63103 USA

[3] St Louis Univ, Dept Biol, St Louis, MO 63103 USA

来源：

INFECTION AND IMMUNITY | 2000年 / 68卷 / 06期

关键词：

D O I：

10.1128/IAI.68.6.3710-3715.2000

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Invasion of enterocytes by Shigella flexneri requires the properly timed release of IpaB and IpaC at the host-pathogen interface; however, only IpaC has been found to possess quantifiable activities in vitro. We demonstrate here that when added to cultured cells, purified IpaC elicits cytoskeletal changes similar to those that occur during Shigella invasion. This IpaC effect mag correlate with its ability to interact with model membranes at physiological pH and to promote entry by an ipaC mutant of S. flexneri.

引用

收藏

页码：3710 / 3715

页数：6

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