Pseudomonas rhodesiae sp nov, a new species isolated from natural mineral waters

Deoxyribonucleic acid relatedness studies (S1 nuclease method) showed that 7 strains of the cluster IIa previously described by Elomari et al. (J. Appl, Bacteriol 78, 71-81, (1995)) formed a homogenous deoxyribonucleic acid hybridization group. This group of strains forms a new species: Pseudomonas rhodesiae sp. nov. A total of 67 strains representing known or partially characterized sprecies of the genus Pseudomonas had 7 to 48% DNA hybridization with Pseudomonas rhodesiae. This new species composed of strains isolated from natural mineral waters, were Gram negative, rod shaped, and motile by means of a single polar flagellum. They were not able to accumulate poly-beta-hydroxybutyrate were capable of respiratory but not fermentative metabolism. They grew at 4 degrees C but not a 41 degrees C, produced fluorescent pigment, catalase, cytochrome oxidase and lecithinase, and possessed arginine dihydrolase system. They did not hydrolyse gelatin and starch, and were able to use glucose, trehalose, 2-ketogluconate, inositol, L-valine and beta-alanine. They possessed L-pyrrolidone arylamidase, L-histidyl-L-serine arylamidase but did not have osidase, esterase = C-12, esterase = C-14, esterase = C-16, and glycyl-L-tryptophane arylamidase. The average guanine + cytosine (G + C) content of the deoxyribonucleic acid was 59 +/- 1 mol%. The type strain of P. rhodesiae has been deposited as CIP 104664(T). The clinical significance of Pseudomonas rhodesiae is unknown.