Basal cells of the human adult airway surface epithelium retain transit-amplifying cell properties

被引:103
作者
Hajj, Rodolphe
Baranek, Thomas
Le Naour, Richard
Lesimple, Pierre
Puchelle, Edith
Coraux, Christelle
机构
[1] CHU Maison Blanche, INSERM, UMRS 514, F-51092 Reims, France
[2] IFR 53, Equipe Associee 3796, Reims, France
关键词
human airway epithelium; basal cells; transit-amplifying cells; CD151; tissue factor;
D O I
10.1634/stemcells.2006-0288
中图分类号
Q813 [细胞工程];
学科分类号
摘要
In numerous airway diseases, such as cystic fibrosis, the epithelium is severely damaged and must regenerate to restore its defense functions. Although the human airway epithelial stem cells have not been identified yet, we have suggested recently that epithelial stem/progenitor cells exist among both human fetal basal and suprabasal cell subsets in the tracheal epithelium. In this study, we analyzed the capacity of human adult basal cells isolated from human adult airway tissues to restore a well-differentiated and functional airway epithelium. To this end, we used the human-specific basal cell markers tetraspanin CD151 and tissue factor (TF) to separate positive basal cells from negative columnar cells with a FACSAria cell sorter. Sorted epithelial cells were seeded into epithelium-denuded rat tracheae that were grafted subcutaneously in nude mice and on collagen-coated porous membranes, where they were grown at the air-liquid interface. Sorted basal and columnar populations were also analyzed for their telomerase activity, a specific transit-amplifying cell marker, by the telomeric repeat amplification protocol assay. After cell sorting, the pure and viable CD151/TF-positive basal cell population proliferated on plastic and adhered on epithelium-denuded rat tracheae, as well as on collagen-coated porous membranes, where it was able to restore a fully differentiated mucociliary and functional airway epithelium, whereas viable columnar negative cells did not. Telomerase activity was detected in the CD151/TF-positive basal cell population, but not in CD151/TF-negative columnar cells. These results demonstrate that human adult basal cells are at least airway surface transit-amplifying epithelial cells.
引用
收藏
页码:139 / 148
页数:10
相关论文
共 64 条
[11]   Dynamic interaction between airway epithelial cells and Staphylococcus aureus [J].
da Silva, MCA ;
Zahm, JM ;
Gras, D ;
Bajolet, O ;
Abely, M ;
Hinnrasky, J ;
Milliot, M ;
de Assis, MC ;
Hologne, C ;
Bonnet, N ;
Merten, M ;
Plotkowski, MC ;
Puchelle, E .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2004, 287 (03) :L543-L551
[12]  
Delplanque A, 2000, J CELL SCI, V113, P767
[13]   TRACHEAL EPITHELIUM - CELL-KINETICS AND DIFFERENTIATION IN NORMAL RAT-TISSUE [J].
DONNELLY, GM ;
HAACK, DG ;
HEIRD, CS .
CELL AND TISSUE KINETICS, 1982, 15 (02) :119-130
[14]   Differentiated and functional human airway epithelium regeneration in tracheal xenografts [J].
Dupuit, F ;
Gaillard, D ;
Hinnrasky, J ;
Mongodin, E ;
De Bentzmann, S ;
Copreni, E ;
Puchelle, E .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2000, 278 (01) :L165-L176
[15]  
ENGELHARDT JF, 1995, DEVELOPMENT, V121, P2031
[16]  
EVANS MJ, 1986, AM J PATHOL, V123, P126
[17]   BASAL CELLS ARE THE PROGENITORS OF PRIMARY TRACHEAL EPITHELIAL-CELL CULTURES [J].
FORD, JR ;
TERZAGHIHOWE, M .
EXPERIMENTAL CELL RESEARCH, 1992, 198 (01) :69-77
[18]   CHARACTERISTICS OF MAGNETICALLY SEPARATED RAT TRACHEAL EPITHELIAL-CELL POPULATIONS [J].
FORD, JR ;
TERZAGHIHOWE, M .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 263 (05) :L568-L574
[19]   Tissue factor and tumor:: Clinical and laboratory aspects [J].
Förster, Y ;
Meye, A ;
Albrecht, S ;
Schwenzer, B .
CLINICA CHIMICA ACTA, 2006, 364 (1-2) :12-21
[20]   Telomerase and differentiation in multicellular organisms: Turn it off, turn it on, and turn it off again [J].
Forsyth, NR ;
Wright, WE ;
Shay, JW .
DIFFERENTIATION, 2002, 69 (4-5) :188-197