Chemo- and enzymatic synthesis of partially and fully N-deacetylated 4-methylumbelliferyl chitobiosides:: Fluorogenic substrates for chitinase

被引：6

作者：

Honda, Y ^{[1
]}

Tanimori, S

Kirihata, M

Kaneko, S

Tokuyasu, K

Hashimoto, M

Watanabe, T

机构：

[1] Osaka Prefecture Univ, Coll Agr, Bioorgan Chem Lab, Sakai, Osaka 5998531, Japan

[2] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

[3] Niigata Univ, Fac Agr, Niigata 9502181, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2000年 / 10卷 / 08期

关键词：

D O I：

10.1016/S0960-894X(00)00116-5

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Partially and fully N-deacetylated 4-methylumberlliferyl chitobioside (1) derivatives, such as GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)(2)-UMB (4), were synthesized using chemo- and enzymatic procedure. Fluorescent aglycon was released from the chitobiosides 1, 2 and 3 by the action of chitinase. These UMB glycosides of hererochitobiose were versatile probes for the investigation of substrate binding chitinase from various sources. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：827 / 829

页数：3

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