Identification of two glutaminases in Rhizobium etli

被引：15

作者：

Duran, S ^{[1
]}

SanchezLinares, L ^{[1
]}

HuertaSaquero, A ^{[1
]}

DuPont, G ^{[1
]}

HuertaZepeda, A ^{[1
]}

Calderon, J ^{[1
]}

机构：

[1] NATL AUTONOMOUS UNIV MEXICO,INST INVEST BIOMED,DEPT BIOTECNOL,MEXICO CITY 04510,DF,MEXICO

来源：

BIOCHEMICAL GENETICS | 1996年 / 34卷 / 11-12期

关键词：

Rhizobium etli; glutaminase; isoenzyme; regulation; glutamine; catabolism; cloning;

D O I：

10.1007/BF00570126

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We present evidence that Rhizobium etli has two glutaminases differentiated by their thermostability and electrophoretic mobility. The thermostable glutaminase (B) is constitutive, in contrast with the thermolabile glutaminase (A), which is positively regulated by glutamine and negatively regulated by ammonium and by the carbon source. In distinction to glutaminase A, glutaminase B plays a minor role in the utilization of glutamine as a carbon sour ce, but it may play a role in maintaining the balance of glutamine and glutamate. By complementation of the Rhizobium etli LM16 mutant that lacks glutaminase A, Mie have cloned the gene that codes for this enzyme.

引用

页码：453 / 465

页数：13