A novel nucleoid-associated protein of Mycobacterium tuberculosis is a sequence homolog of GroEL

被引:50
作者
Basu, Debashree [1 ]
Khare, Garima [2 ]
Singh, Shashi [3 ]
Tyagi, Anil [2 ]
Khosla, Sanjeev [4 ]
Mande, Shekhar C. [1 ]
机构
[1] Ctr DNA Fingerprinting & Diagnost, Struct Biol Lab, Hyderabad 500001, Andhra Pradesh, India
[2] Univ Delhi, Dept Biochem, New Delhi 110021, India
[3] Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India
[4] Ctr DNA Fingerprinting & Diagnost, Mammalian Genet Lab, Hyderabad 500001, Andhra Pradesh, India
基金
英国惠康基金;
关键词
DNA-BINDING PROTEIN; SINGLE-STRANDED-DNA; ESCHERICHIA-COLI; H-NS; BACTERIAL CHROMATIN; IDENTIFICATION; RECOGNITION; COMPACTION; MECHANISM; EVOLUTION;
D O I
10.1093/nar/gkp502
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Mycobacterium tuberculosis genome sequence reveals remarkable absence of many nucleoid-associated proteins (NAPs), such as HNS, Hfq or DPS. In order to characterize the nucleoids of M. tuberculosis, we have attempted to identify NAPs, and report an interesting finding that a chaperonin-homolog, GroEL1, is nucleoid associated. We report that M. tuberculosis GroEL1 binds DNA with low specificity but high affinity, suggesting that it might have naturally evolved to bind DNA. We are able to demonstrate that GroEL1 can effectively function as a DNA-protecting agent against DNase I or hydroxyl-radicals. Moreover, Atomic Force Microscopic studies reveal that GroEL1 can condense a large DNA into a compact structure. We also provide in vivo evidences that include presence of GroEL1 in purified nucleoids, in vivo crosslinking followed by Southern hybridizations and immunofluorescence imaging in M. tuberculosis confirming that GroEL1: DNA interactions occur in natural biological settings. These findings therefore reveal that M. tuberculosis GroEL1 has evolved to be associated with nucleoids.
引用
收藏
页码:4944 / 4954
页数:11
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