Solid-State 13C NMR Study of a Composite of Tobacco Xyloglucan and Gluconacetobacter xylinus Cellulose: Molecular Interactions between the Component Polysaccharides

被引:18
作者
Bootten, Tracey J. [1 ]
Harris, Philip J. [1 ]
Melton, Laurence D. [2 ]
Newman, Roger H. [3 ]
机构
[1] Univ Auckland, Sch Biol Sci, Auckland 1, New Zealand
[2] Univ Auckland, Dept Chem, Auckland 1, New Zealand
[3] Ind Res Ltd, Lower Hutt, New Zealand
关键词
PRIMARY-CELL WALLS; NUCLEAR-MAGNETIC-RESONANCE; STRUCTURAL-ANALYSIS; SPECTROSCOPIC METHODS; TAMARIND XYLOGLUCAN; CRYSTALLINE FORMS; OLIGOSACCHARIDES; POLYMERS; NOMENCLATURE; DIGESTION;
D O I
10.1021/bm900762m
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
To investigate possible molecular interactions between xyloglucans (XGs) and cellulose in plant cell walls, a model composite was produced using cellulose from the bacterium Gluconacetobacter xylinus and XG from the walls of a tobacco cell-suspension culture that had been incubated with C-13-labeled glucose. Solid-state C-13 NMR with cross-polarization (CP) and magic-angle spinning (MAS) was used in combination with proton spin-relaxation editing to separate signals from crystalline (rigid) and less rigid domains of the composite. Signals from XG were confined to subspectra of less rigid domains, with no detectable signals from XG attached to surfaces of cellulose crystallites. Signal displacements indicated XGs were more rigid than the mobile coil (twisted backbone) conformation expected for unattached XGs. Similar C-13 chemical shifts were observed in a single-pulse excitation experiment. The results were not compatible with extensive hydrogen bonding between XG and cellulose, but were consistent with a composite structure in which cellulose crystallites were embedded in a matrix of XG with a semirigid (straightened backbone) conformation, that is, a matrix that is partly ordered rather than amorphous.
引用
收藏
页码:2961 / 2967
页数:7
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