Evaluation of the ALiPHAT Method for PC-IDMS and Correlation of Limits-of-Detection with Nonpolar Surface Area

被引：15

作者：

Williams, D. Keith, Jr. ^{[1
]}

Comins, Daniel L. ^{[1
]}

Whitten, Jerry L. ^{[1
]}

Muddiman, David C. ^{[1
]}

机构：

[1] N Carolina State Univ, Dept Chem, WM Keck FT ICR Mass Spectrometry Lab, Raleigh, NC 27695 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2009年 / 20卷 / 11期

关键词：

DILUTION MASS-SPECTROMETRY; PROTEOME ANALYSIS; ABSOLUTE QUANTIFICATION; QUANTITATIVE-ANALYSIS; SMALL PEPTIDES; DERIVATIZATION; MIXTURES; PROTEINS; ENERGY; TAGS;

D O I：

10.1016/j.jasms.2009.07.019

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

PC-IDMS experiments for two peptides, laminin nonapeptide and the N-terminal tryptic peptide of prostate specific antigen, were performed utilizing a variety of alkylating reagents. These experiments were conducted to investigate how hydrophobicity influences the limits-of-detection (LOD) by altering their electrospray ionization response. Nonpolar surface areas were calculated for both peptides and all alkylating reagents to provide an estimate of the hydrophobicity of the differently alkylated peptides. Decreases in LOD by 2-fold were observed for both peptides between the best and worst performing combination of alkylating reagent. However, while an increase in hydrophobicity was found to aid in decreasing LOD to an extent, beyond a certain hydrophobicity, we observed a decrease. (J Am Soc Mass Spectrom 2009, 20, 2006-2012) (C) 2009 American Society for Mass Spectrometry

引用

页码：2006 / 2012

页数：7

共 33 条

[1] Absolute quantification of the model biomarker prostate-specific antigen in serum by LC-MS/MS using protein cleavage and isotope dilution mass spectrometry [J].

Barnidge, DR ;

Goodmanson, MK ;

Klee, GG ;

Muddiman, DC .

JOURNAL OF PROTEOME RESEARCH, 2004, 3 (03) :644-652

[2]

Barr JR, 1996, CLIN CHEM, V42, P1676

[3] De novo peptide sequencing and quantitative profiling of complex protein mixtures using mass-coded abundance tagging [J].

Cagney, G ;

Emili, A .

NATURE BIOTECHNOLOGY, 2002, 20 (02) :163-170

[4] Relating electrospray ionization response to nonpolar character of small peptides [J].

Cech, NB ;

Enke, CG .

ANALYTICAL CHEMISTRY, 2000, 72 (13) :2717-2723

[5] Practical implications of some recent studies in electrospray ionization fundamentals [J].

Cech, NB ;

Enke, CG .

MASS SPECTROMETRY REVIEWS, 2001, 20 (06) :362-387

[6] Global internal standard technology for comparative proteomics [J].

Chakraborty, A ;

Regnier, FE .

JOURNAL OF CHROMATOGRAPHY A, 2002, 949 (1-2) :173-184

[7] APPLICATIONS OF ISOTOPE-DILUTION MASS-SPECTROMETRY IN CLINICAL-CHEMISTRY, PHARMACOKINETICS, AND TOXICOLOGY [J].

DELEENHEER, AP ;

THIENPONT, LM .

MASS SPECTROMETRY REVIEWS, 1992, 11 (04) :249-307

[8] ION FORMATION FROM CHARGED DROPLETS - ROLES OF GEOMETRY, ENERGY, AND TIME [J].

FENN, JB .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (07) :524-535

[9] Derivatisation of arginine residues with malondialdehyde for the analysis of peptides and protein digests by LC-ESI-MSMS [J].

Foettinger, Alexandra ;

Leitner, Alexander ;

Lindner, Wolfgang .

JOURNAL OF MASS SPECTROMETRY, 2006, 41 (05) :623-632

[10] Achieving augmented limits of detection for peptides with hydrophobic alkyl tags [J].

Frahm, Jennifer L. ;

Bori, Ibrahim D. ;

Comins, Daniel L. ;

Hawkridge, Adam M. ;

Muddiman, David C. .

ANALYTICAL CHEMISTRY, 2007, 79 (11) :3989-3995

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