Detection of DNA using bioactive paper strips

被引：92

作者：

Ali, M. Monsur ^{[1
,2
]}

Aguirre, Sergio D. ^{[1
,2
]}

Xu, Yaqin ^{[3
]}

Filipe, Carlos D. M. ^{[3
]}

Pelton, Robert ^{[3
]}

Li, Yingfu ^{[1
,2
]}

机构：

[1] McMaster Univ, Dept Biochem & Biomed Sci, Hamilton, ON L8N 3Z5, Canada

[2] McMaster Univ, Dept Chem, Hamilton, ON L8N 3Z5, Canada

[3] McMaster Univ, Dept Chem Engn, Hamilton, ON L8S 4L7, Canada

来源：

CHEMICAL COMMUNICATIONS | 2009年 / 43期

基金：

加拿大创新基金会; 加拿大自然科学与工程研究理事会;

关键词：

ROLLING-CIRCLE AMPLIFICATION; MICROFLUIDIC DEVICES; CLINICAL UTILITY; BIOASSAYS; OLIGONUCLEOTIDES; MICROARRAYS; MICROGEL; PEPTIDE; ENZYME; PROBES;

D O I：

10.1039/b911559e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Paper strips containing DNA-conjugated microgels (MG) are used to achieve sensitive DNA detection in three steps: target DNA promoted ligation of a DNA primer to the MG-bound DNA, rolling circle amplification (RCA) between the primer and a circle DNA, and hybridization of the RCA products and a fluorescent DNA probe.

引用

页码：6640 / 6642

页数：3

共 33 条

[1] Making more of DNA - Cheap and efficient DNA amplifying method relies on colloid support
Mearns, Freya
[J]. PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES, 2007, 6 (11) : B83 - B83
[2] Ligation-mediated rolling-circle amplification-based approaches to single nucleotide polymorphism detection
Bakht, S
Qi, XQ
[J]. EXPERT REVIEW OF MOLECULAR DIAGNOSTICS, 2005, 5 (01) : 111 - 116
[3] Array-based DNA diagnostics: Let the revolution begin
Beaudet, Arthur L.
Belmont, John W.
[J]. ANNUAL REVIEW OF MEDICINE, 2008, 59 : 113 - 129
[4] Bianchessi M, 2008, TUMORI J, V94, P216
[5] Fabrication of a modular tissue construct in a microfluidic chip
Bruzewicz, Derek A.
McGuigan, Alison P.
Whitesides, George M.
[J]. LAB ON A CHIP, 2008, 8 (05) : 663 - 671
[6] Low-cost printing of poly(dimethylsiloxane) barriers to define microchannels in paper
Bruzewicz, Derek A.
Reches, Meital
Whitesides, George M.
[J]. ANALYTICAL CHEMISTRY, 2008, 80 (09) : 3387 - 3392
[7] A systematic review of the clinical validity and clinical utility of DNA testing for hereditary haemochromatosis type 1 in at-risk populations
Bryant, J.
Cooper, K.
Picot, J.
Clegg, A.
Roderick, P.
Rosenberg, W.
Patch, C.
[J]. JOURNAL OF MEDICAL GENETICS, 2008, 45 (08) : 513 - 518
[8] Diagnosing viruses by the rolling circle amplified synthesis of DNAzymes
Cheglakov, Zoya
Weizmann, Yossi
Basnar, Bernhard
Willner, Itamar
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2007, 5 (02) : 223 - 225
[9] Demidov Vadim V, 2002, Expert Rev Mol Diagn, V2, P542, DOI 10.1586/14737159.2.6.542
[10] ROLLING REPLICATION OF SHORT DNA CIRCLES
FIRE, A
XU, SQ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (10) : 4641 - 4645

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