A new mouse model for the trisomy of the Abcg1-U2af1 region reveals the complexity of the combinatorial genetic code of down syndrome

被引：88

作者：

Pereira, Patricia Lopes ^{[2
]}

Magnol, Laetitia ^{[2
]}

Sahun, Ignasi ^{[3
]}

Brault, Veronique ^{[2
]}

Duchon, Arnaud ^{[2
]}

Prandini, Paola ^{[4
]}

Gruart, Agnes ^{[5
]}

Bizot, Jean-Charles ^{[6
]}

Chadefaux-Vekemans, Bernadette ^{[7
]}

Deutsch, Samuel ^{[4
]}

Trovero, Fabrice ^{[6
]}

Delgado-Garcia, Jose Maria ^{[5
]}

Antonarakis, Stylianos E. ^{[4
]}

Dierssen, Mara ^{[3
]}

Herault, Yann ^{[1
,2
]}

机构：

[1] Univ Orleans, CNRS, UPS44, IEM,TAAM,Inst Transgenose,UMR6218, F-45071 Orleans 2, France

[2] CNRS, MIE, UMR6218, F-45071 Orleans 2, France

[3] Dr Aiguader 88 & CIBER Enfermedades Raras, Ctr Genom Regulat, Genes & Dis Program, Barcelona 08003, Spain

[4] Univ Geneva, Sch Med, Dept Genet Med & Dev, CH-1211 Geneva, Switzerland

[5] Univ Pablo de Olavide, Div Neurociencias, Seville, Spain

[6] Key Obs SA, F-45100 Orleans, France

[7] Univ Paris 05, Fac Med, Hop Necker Enfants Malad, Serv Biochim Metab, F-75730 Paris, France

来源：

HUMAN MOLECULAR GENETICS | 2009年 / 18卷 / 24期

关键词：

LONG-TERM POTENTIATION; TS65DN MOUSE; SYNAPTIC PLASTICITY; TS1CJE MOUSE; CEREBELLAR TRANSCRIPTOME; EXPRESSION VARIATION; SYNDROME PHENOTYPES; MENTAL-RETARDATION; CA3-CA1; SYNAPSE; MEMORY;

D O I：

10.1093/hmg/ddp438

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mental retardation in Down syndrome (DS), the most frequent trisomy in humans, varies from moderate to severe. Several studies both in human and based on mouse models identified some regions of human chromosome 21 (Hsa21) as linked to cognitive deficits. However, other intervals such as the telomeric region of Hsa21 may contribute to the DS phenotype but their role has not yet been investigated in detail. Here we show that the trisomy of the 12 genes, found in the 0.59 Mb (Abcg1-U2af1) Hsa21 sub-telomeric region, in mice (Ts1Yah) produced defects in novel object recognition, open-field and Y-maze tests, similar to other DS models, but induces an improvement of the hippocampal-dependent spatial memory in the Morris water maze along with enhanced and longer lasting long-term potentiation in vivo in the hippocampus. Overall, we demonstrate the contribution of the Abcg1-U2af1 genetic region to cognitive defect in working and short-term recognition memory in DS models. Increase in copy number of the Abcg1-U2af1 interval leads to an unexpected gain of cognitive function in spatial learning. Expression analysis pinpoints several genes, such as Ndufv3, Wdr4, Pknox1 and Cbs, as candidates whose overexpression in the hippocampus might facilitate learning and memory in Ts1Yah mice. Our work unravels the complexity of combinatorial genetic code modulating different aspect of mental retardation in DS patients. It establishes definitely the contribution of the Abcg1-U2af1 orthologous region to the DS etiology and suggests new modulatory pathways for learning and memory.

引用

页码：4756 / 4769

页数：14