Strategies for targeted nonviral delivery of siRNAs in vivo

被引：90

作者：

Kim, Sang-Soo ^{[1
]}

Garg, Himanshu ^{[1
]}

Joshi, Anjali ^{[1
]}

Manjunath, N. ^{[1
]}

机构：

[1] Texas Tech Univ, Hlth Sci Ctr, Paul L Foster Sch Med, Dept Biomed Sci,Ctr Excellence Infect Dis, El Paso, TX 79905 USA

来源：

TRENDS IN MOLECULAR MEDICINE | 2009年 / 15卷 / 11期

关键词：

SMALL INTERFERING RNA; INHIBITS TUMOR-GROWTH; GENE-THERAPY; CELLULAR UPTAKE; PROTECTS MICE; SYSTEM; CELLS; POLYETHYLENIMINE; THERAPEUTICS; MOUSE;

D O I：

10.1016/j.molmed.2009.09.001

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Silencing specific gene expression by RNA interference (RNAi) has rapidly become a standard tool for the reverse genetic analysis of gene functions. It also has tremendous potential for managing diseases for which effective treatment is currently unavailable or suboptimal. However, the poor cellular uptake of synthetic small interfering RNAs (siRNAs) is a major impediment for their clinical use. Great progress has been made in recent years to overcome this barrier, and several methods have been described for the in vivo delivery of siRNA. Moreover, the latest advances have focused on achieving targeted siRNA delivery restricted to relevant tissues and cell types in vivo. These approaches are expected to reduce the dose requirement as well as minimize siRNA-induced toxicities, thereby advancing the field of siRNA therapy towards clinical use.

引用

页码：491 / 500

页数：10

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