Engineering embryonic stem cells with recombinase systems

被引：20

作者：

Schnuetgen, Frank ^{[1
]}

Stewart, A. Francis

von Melchner, Harald

Anastassiadis, Konstantinos

机构：

[1] Goethe Univ Frankfurt, Sch Med, Dept Mol Hematol, Frankfurt, Germany

[2] Tech Univ Dresden, D-8027 Dresden, Germany

来源：

STEM CELL TOOLS AND OTHER EXPERIMENTAL PROTOCOLS | 2006年 / 420卷

关键词：

D O I：

10.1016/S0076-6879(06)20007-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The combined use of site-specific recombination and gene targeting or trapping in embryonic stem cells (ESCs) has resulted in the emergence of technologies that enable the induction of mouse mutations in a prespecified temporal and spatially restricted manner. Their large-scale implementation by several international mouse mutagenesis programs will lead to the assembly of a library of ES cell lines harboring conditional mutations in every single gene of the mouse genome. In anticipation of this unprecedented resource, this chapter will focus on site-specific recombination strategies and issues pertinent to ESCs and mice. The upcoming ESC resource and the increasing sophistication of site-specific recombination technologies will greatly assist the functional annotation of the human genome and the animal modeling of human disease.

引用

页码：100 / 136

页数：37

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