High-yield activation of scaffold polymer surfaces to attach cell adhesion molecules

被引：24

作者：

Dennes, T. Joseph ^{[1
]}

Hunt, Geoffrey C.

Schwarzbauer, Jean E.

Schwartz, Jeffrey

机构：

[1] Princeton Univ, Dept Chem, Princeton, NJ 08544 USA

[2] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 01期

关键词：

D O I：

10.1021/ja065217t

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Zirconium tetra(tert-butoxide) reacts with surface amide groups of polyamide nylon 6/6 to give (eta(2)-amidate) zirconium complexes in high yield. These surface complexes react to bond the cell-adhesive peptide arginine-glycine-aspartic acid (RGD) to the polymer surface. A surface loading of 0.18 nmol/cm(2) of RGD is achieved, which is 20-1000 times higher than previously reported attainable on natural or synthetic polymers by other strategies. Approximately 40% of the nylon surface is covered by the RGD, which gives a surface that is both stable to hydrolysis and highly active for cell adhesion and spreading in vitro.

引用

页码：93 / 97

页数：5

共 32 条

[1] Human bone marrow stromal cell and ligament fibroblast responses on RGD-modified silk fibers [J].

Chen, JS ;

Altman, GH ;

Karageorgiou, V ;

Horan, R ;

Collette, A ;

Volloch, V ;

Colabro, T ;

Kaplan, DL .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2003, 67A (02) :559-570

[2] TISSUE ENGINEERING BY CELL TRANSPLANTATION USING DEGRADABLE POLYMER SUBSTRATES [J].

CIMA, LG ;

VACANTI, JP ;

VACANTI, C ;

INGBER, D ;

MOONEY, D ;

LANGER, R .

JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME, 1991, 113 (02) :143-151

[3]

Cook AD, 1997, J BIOMED MATER RES, V35, P513

[4] Self-assembled monolayers of α,ω-diphosphonic acids on Ti enable complete or spatially controlled surface derivatization [J].

Danahy, MP ;

Avaltroni, MJ ;

Midwood, KS ;

Schwarzbauer, JE ;

Schwartz, J .

LANGMUIR, 2004, 20 (13) :5333-5337

[5] A modular and supramolecular approach to bioactive scaffolds for tissue engineering [J].

Dankers, PYW ;

Harmsen, MC ;

Brouwer, LA ;

Van Luyn, MJA ;

Meijer, EW .

NATURE MATERIALS, 2005, 4 (07) :568-574

[6] Generation of cell adhesive substrates using peptide fluoralkyl surface modifiers [J].

Ernsting, MJ ;

Bonin, GC ;

Yang, ML ;

Labow, RS ;

Santerre, JP .

BIOMATERIALS, 2005, 26 (33) :6536-6546

[7] Organometallic chemistry of amidate complexes.: Accelerating effect of bidentate ligands on the reductive elimination of N-aryl amidates from palladium(II) [J].

Fujita, Ken-ichi ;

Yamashita, Makoto ;

Puschmann, Florian ;

Alvarez-Falcon, Miguel Martinez ;

Incarvito, Christopher D. ;

Hartwig, John F. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2006, 128 (28) :9044-9045

[8] Self-assembly and bonding of alkanephosphonic acids on the native oxide surface of titanium [J].

Gawalt, ES ;

Avaltroni, MJ ;

Koch, N ;

Schwartz, J .

LANGMUIR, 2001, 17 (19) :5736-5738

[9] Bonding organics to Ti alloys: Facilitating human osteoblast attachment and spreading on surgical implant materials [J].

Gawalt, ES ;

Avaltroni, MJ ;

Danahy, MP ;

Silverman, BM ;

Hanson, EL ;

Midwood, KS ;

Schwarzbauer, JE ;

Schwartz, J .

LANGMUIR, 2003, 19 (01) :200-204

[10] RGD modified polymers: biomaterials for stimulated cell adhesion and beyond [J].

Hersel, U ;

Dahmen, C ;

Kessler, H .

BIOMATERIALS, 2003, 24 (24) :4385-4415

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