Chemical imaging of microfluidic flows using ATR-FTIR spectroscopy

被引：109

作者：

Chan, K. L. Andrew ^{[1
]}

Gulati, Shelly ^{[2
,3
]}

Edel, Joshua B. ^{[2
,3
]}

de Mello, Andrew J. ^{[2
]}

Kazarian, Sergei G. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem Engn, London SW7 2AZ, England

[2] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[3] Univ London Imperial Coll Sci Technol & Med, Inst Biomed Engn, London SW7 2AZ, England

来源：

LAB ON A CHIP | 2009年 / 9卷 / 20期

基金：

英国工程与自然科学研究理事会; 欧洲研究理事会;

关键词：

SYSTEMS;

D O I：

10.1039/b909573j

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Elucidating the chemical composition of microfluidic flows is crucial in both understanding and optimising reactive processes within small-volume environments. Herein we report the implementation of a novel detection methodology based on Attenuated Total Reflection (ATR)-Fourier Transform Infra-Red (FTIR) spectroscopic imaging using an infrared focal plane array detector for microfluidic applications. The method is based on the combination of an inverted prism-shape ATR crystal with a poly(dimethylsiloxane)-based microfluidic mixing device. To demonstrate the efficacy of this approach, we report the direct measurement and imaging of the mixing of two liquids of different viscosities and the imaging and mixing of H2O and D2O with consecutive H/D isotope exchange. This chemically specific imaging approach allows direct analysis of fluid composition as a function of spatial position without the use of added labels or dyes, and can be used to study many processes in microfluidics ranging from reactions to separations.

引用

页码：2909 / 2913

页数：5

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