Mammalian two-hybrids come of age

被引：45

作者：

Lievens, Sam ^{[1
,2
]}

Lemmens, Irma ^{[1
,2
]}

Tavernier, Jan ^{[1
,2
]}

机构：

[1] VIB, Dept Med Prot Res, B-9000 Ghent, Belgium

[2] Univ Ghent, Dept Biochem, B-9000 Ghent, Belgium

来源：

TRENDS IN BIOCHEMICAL SCIENCES | 2009年 / 34卷 / 11期

关键词：

PROTEIN-PROTEIN INTERACTION; FRAGMENT COMPLEMENTATION ASSAYS; INTERACTION NETWORK; DRUG DISCOVERY; IN-VITRO; RECEPTOR; CELLS; TRAP; VISUALIZATION; MICROARRAYS;

D O I：

10.1016/j.tibs.2009.06.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A diverse series of mammalian two-hybrid technologies for the detection of protein-protein interactions have emerged in the past few years, complementing the established yeast two-hybrid approach. Given the mammalian background in which they operate, these assays open new avenues to study the dynamics of mammalian protein interaction networks, i.e. the temporal, spatial and functional modulation of protein-protein associations. In addition, novel assay formats are available that enable high-throughput mammalian two-hybrid applications, facilitating their use in large-scale interactome mapping projects. Finally, as they can be applied in drug discovery and development programs, these techniques also offer exciting new opportunities for biomedical research.

引用

页码：579 / 588

页数：10

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