Edible seaweed as future functional food: Identification of α-glucosidase inhibitors by combined use of high-resolution α-glucosidase inhibition profiling and HPLC-HRMS-SPE-NMR

被引：80

作者：

Liu, Bingrui ^{[1
]}

Kongstad, Kenneth T. ^{[1
]}

Wiese, Stefanie ^{[2
]}

Jager, Anna K. ^{[1
]}

Staerk, Dan ^{[1
]}

机构：

[1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Drug Design & Pharmacol, Univ Pk 2, DK-2100 Copenhagen, Denmark

[2] Univ Copenhagen, Fac Life Sci, Dept Basic Sci & Environm, Thorvaldsensvej 40, DK-1871 Frederiksberg C, Denmark

来源：

FOOD CHEMISTRY | 2016年 / 203卷

关键词：

alpha-Glucosidase inhibition; Antioxidant capacity; Edible seaweed; Functional food; High-resolution inhibition profile; HPLC-HRMS-SPE-NMR; MAGNETIC-RESONANCE-SPECTROSCOPY; POLYUNSATURATED FATTY-ACIDS; PROOF-OF-CONCEPT; ANTIOXIDANT PROPERTIES; CRUDE EXTRACT; BROWN; CONSTITUENTS; AMYLASE; ASSAY; MACROALGAE;

D O I：

10.1016/j.foodchem.2016.02.001

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Crude chloroform, ethanol and acetone extracts of nineteen seaweed species were screened for their antioxidant and alpha-glucosidase inhibitory activity. Samples showing more than 60% alpha-glucosidase inhibitory activity, at a concentration of 1 mg/ml, were furthermore investigated using high-resolution alpha-glucosidase inhibition profiling combined with high-performance liquid chromatography-high-resolu tion mass spectrometry-solid-phase extraction-nuclear magnetic resonance spectroscopy (HR-bioas say/HPLC-HRMS-SPE-NMR). The results showed Ascophyllum nodosum and Fucus vesicolosus to be rich in antioxidants, equaling a Trolox equivalent antioxidant capacity of 135 and 108 mM Trolox mg(-1) extract, respectively. HR-bioassay/HPLC-HRMS-SPE-NMR showed the alpha-glucosidase inhibitory activity of A. nodosum, F. vesoculosus, Laminaria digitata, Laminaria japonica and Undaria pinnatifida to be caused by phlorotannins as well as fatty acids - with oleic acid, linoleic acid and eicosapentaenoic acid being the most potent with IC50 values of 0.069, 0.075 and 0.10 mM, respectively, and showing a mixed-type inhibition mode. (c) 2016 Elsevier Ltd. All rights reserved.

引用

页码：16 / 22

页数：7

共 40 条

[1]

In vitro antioxidant properties of crude extracts and compounds from brown algae [J].