Ap(3)A and Ap(4)A are primers for oligoadenylate synthesis catalyzed by interferon-inducible 2-5A synthetase

被引:25
作者
Turpaev, K
Hartmann, R
Kisselev, L
Justesen, J
机构
[1] AARHUS UNIV,DEPT MOL & STRUCT BIOL,AARHUS,DENMARK
[2] RUSSIAN ACAD SCI,VA ENGELHARDT MOL BIOL INST,MOSCOW,RUSSIA
基金
俄罗斯基础研究基金会;
关键词
tryptophanyl tRNA synthetase; 2-5A synthetase; AP(3)A; Ap(4)A;
D O I
10.1016/S0014-5793(97)00365-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The biological role of Ap(3)A synthesized in cells by tryptophanyl-tRNA synthetase (WRS) is unknown. Previously rue have demonstrated that the cellular level of Ap(3)A significantly increases after interferon treatment. Here we show that the human 46 kDa 2-5A synthetase efficiently utilizes Ap(3)A as a primer for oligoadenylate synthesis. The K-m for Ap(3)A is several-fold lower than for Ap(4)A and 100-fold lower than for ATP. This implies that Ap(3)A might be a natural primer for the 2'-adenylation reaction catalysed by 2-5A synthetase. Since WRS and 2-5A synthetase are both interferon-inducible proteins, a new link between two interferon-dependent enzymes is established. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:177 / 181
页数:5
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