Ap(3)A and Ap(4)A are primers for oligoadenylate synthesis catalyzed by interferon-inducible 2-5A synthetase

被引：25

作者：

Turpaev, K

Hartmann, R

Kisselev, L

Justesen, J

机构：

[1] AARHUS UNIV,DEPT MOL & STRUCT BIOL,AARHUS,DENMARK

[2] RUSSIAN ACAD SCI,VA ENGELHARDT MOL BIOL INST,MOSCOW,RUSSIA

来源：

FEBS LETTERS | 1997年 / 408卷 / 02期

基金：

俄罗斯基础研究基金会;

关键词：

tryptophanyl tRNA synthetase; 2-5A synthetase; AP(3)A; Ap(4)A;

D O I：

10.1016/S0014-5793(97)00365-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The biological role of Ap(3)A synthesized in cells by tryptophanyl-tRNA synthetase (WRS) is unknown. Previously rue have demonstrated that the cellular level of Ap(3)A significantly increases after interferon treatment. Here we show that the human 46 kDa 2-5A synthetase efficiently utilizes Ap(3)A as a primer for oligoadenylate synthesis. The K-m for Ap(3)A is several-fold lower than for Ap(4)A and 100-fold lower than for ATP. This implies that Ap(3)A might be a natural primer for the 2'-adenylation reaction catalysed by 2-5A synthetase. Since WRS and 2-5A synthetase are both interferon-inducible proteins, a new link between two interferon-dependent enzymes is established. (C) 1997 Federation of European Biochemical Societies.

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页码：177 / 181

页数：5

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