Response surface analysis of lipase production by freely suspended Rhizopus arrhizus

被引：66

作者：

Elibol, M ^{[1
]}

Ozer, D ^{[1
]}

机构：

[1] Firat Univ, Dept Chem Engn, TR-23119 Elazig, Turkey

来源：

PROCESS BIOCHEMISTRY | 2002年 / 38卷 / 03期

关键词：

lipase; Rhizopus arrhizus; response surface methodology; glucose; inducer (corn oil);

D O I：

10.1016/S0032-9592(02)00076-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The combined effects of initial glucose concentration and inducer (corn oil) concentration on lipase production by freely suspended Rhizopus arrhizus were investigated using response surface methodology (RSM). A 2(2) full-factorial central composite design was employed for experimental design and analysis of the results. The optimum glucose and inducer concentrations were found to be 1.1 and 3.3 g/l, respectively. In these conditions, the biomass concentration of 2.4 g/l with a lipolytic activity of 370 mumol/l min was attained. These results are in close agreement with the model parameters. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：367 / 372

页数：6

共 17 条

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