Application of a homogenous assay for the detection of 2,4,6-trinitrotoluene to environmental water samples

被引：5

作者：

Goldman, Ellen R. ^{[1
]}

Egge, Adrienne L. ^{[1
]}

Medintz, Igor L. ^{[1
]}

Lassman, Michael E. ^{[1
]}

Anderson, George P. ^{[1
]}

机构：

[1] USN, Res Lab, Ctr Biomol Sci & Engn, Washington, DC 20375 USA

来源：

THESCIENTIFICWORLDJOURNAL | 2005年 / 5卷

关键词：

homogeneous assay; 2,4,6-trinitrotoluene ( TNT); environmental monitoring; explosives; fluorescent displacement immunoassay;

D O I：

10.1100/tsw.2005.58

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A homogeneous assay was used to detect 2,4,6-trinitrotoluene (TNT) spiked into environmental water samples. This assay is based on changes in fluorescence emission intensity when TNT competitively displaces a fluorescently labeled, TNT analog bound to an anti-TNT antibody. The effectiveness of the assay was highly dependent on the source of the sample being tested. As no correlation between pH and assay performance was observed, ionic strength was assumed to be the reason for variation in assay results. Addition of 10x phosphate-buffered saline to samples to increase their ionic strength to that of our standard laboratory buffer (about 0.17 M) significantly improved the range over which the assay functioned in several river water samples.

引用

页码：446 / 451

页数：6

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