Metabolic phenotyping of genetically modified mice: An NMR metabonomic approach

被引：27

作者：

Rull, Anna ^{[1
]}

Vinaixa, Maria ^{[2
]}

Angel Rodriguez, Miguel ^{[2
]}

Beltran, Raul ^{[1
]}

Brezmes, Jesus ^{[2
]}

Canellas, Nicolau ^{[2
]}

Correig, Xavier ^{[2
]}

Joven, Jorge ^{[1
]}

机构：

[1] Univ Rovira & Virgili, IISPV, Hosp Univ St Joan Reus, Ctr Recerca Biomed, Reus 43201, Spain

[2] Univ Rovira & Virgili, Tarragona 43007, Spain

来源：

BIOCHIMIE | 2009年 / 91卷 / 08期

关键词：

CYP7A1; H-1 NMR spectroscopy; MCP-1; Metabonomics; Taurine; MONOCYTE CHEMOATTRACTANT PROTEIN-1; DEFICIENT MICE; TAURINE; LIVER; CHOLESTEROL; OBESITY;

D O I：

10.1016/j.biochi.2009.04.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Monocyte chemoattractant protein-1 (MCP-1) plays a relevant role in macrophage migration but recent findings suggest an additional role in lipid and glucose metabolism. We report the use of H-1 NMR spectroscopy as a useful complementary method to assess the metabolic function of this gene in a comparative strategy. This metabonomic analysis was rapid, simple, quantitative and reproducible, and revealed a suggestive relationship between the expression of the MCP-1 gene and hepatic glucose and taurine concentrations. This approach should be considered in genetically modified mice when a metabolic alteration is suspected, or in routine assessment of metabolic phenotype. (C) 2009 Elsevier Masson SAS. All rights reserved.

引用

页码：1053 / 1057

页数：5

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