Modulation of ATP-dependent chromatin-remodeling complexes by inositol polyphosphates

被引：274

作者：

Shen, XT ^{[1
]}

Xiao, H ^{[1
]}

Ranallo, R ^{[1
]}

Wu, WH ^{[1
]}

Wu, C ^{[1
]}

机构：

[1] NCI, Ctr Canc Res, Mol Cell Biol Lab, NIH, Bethesda, MD 20892 USA

来源：

SCIENCE | 2003年 / 299卷 / 5603期

关键词：

D O I：

10.1126/science.1078068

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Eukaryotes use adenosine triphosphate (ATP)-dependent chromatin-remodeling complexes to regulate gene expression. Here, we show that inositol polyphosphates can modulate the activities of several chromatin-remodeling complexes in vitro. Inositol hexakisphosphate (IP6) inhibits nucleosome mobilization by NURF, ISW2, and INO80 complexes. In contrast, nucleosome mobilization by the yeast SWI/SNF complex is stimulated by inositol tetrakisphosphate (IP4) and inositol pentakisphosphate (IP5). We demonstrate that mutations in genes encoding inositol polyphosphate kinases that produce IP4, IP5, and IP6 impair transcription in vivo. These results provide a link between inositol polyphosphates, chromatin remodeling, and gene expression.

引用

页码：112 / 114

页数：4

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