Identification and characterisation of a novel conserved outer membrane protein from Neisseria meningitidis

被引：12

作者：

Peak, IRA

Srikhanta, Y

Dieckelmann, M

Moxon, ER

Jennings, MP ^{[1
]}

机构：

[1] Univ Queensland, Dept Microbiol & Parasitol, Brisbane, Qld 4072, Australia

[2] John Radcliffe Hosp, Inst Mol Med, Dept Paediat, Mol Infect Dis Grp, Oxford OX3 9DU, England

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 2000年 / 28卷 / 04期

关键词：

Neisseria meningitidis; outer membrane protein; high molecular mass; adhesin;

D O I：

10.1111/j.1574-695X.2000.tb01494.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We have identified a homologue of the adhesin AIDA-I of Escherichia coli in Neisseria meningitidis. This gene was designated nhhA (Neisseria hia homologue), as analysis of the complete coding sequence revealed that it is more closely related to the: adhesins Hia and Hsf of Haemophilus influenzae. The sequence of nhhA was determined from 10 strains, and found to be highly conserved. Studies of the localisation by Western immunoblot analysis of total cell proteins and outer membrane complex preparations and by immunogold electron microscopy revealed that NhhA is located in the outer membrane. A strain survey showed that nhhA is present in 85/85 strains of N. meningitidis representative of all the major disease-associated serogroups, based on Southern blot analysis. It is expressed in the majority of strains tested by Western immunoblot. (C) 2000 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.

引用

收藏

页码：329 / 334

页数：6

相关论文

共 19 条

[1] A protective epitope of Moraxella catarrhalis is encoded by two different genes [J].

Aebi, C ;

Maciver, I ;

Latimer, JL ;

Cope, LD ;

Stevens, MK ;

Thomas, SE ;

McCracken, GH ;

Hansen, EJ .

INFECTION AND IMMUNITY, 1997, 65 (11) :4367-4377

[2] Identification of a second family of high-molecular-weight adhesion proteins expressed by non-typable Haemophilus influenzae [J].

Barenkamp, SJ ;

StGeme, JW .

MOLECULAR MICROBIOLOGY, 1996, 19 (06) :1215-1223

[3] CLONING, EXPRESSION, AND DNA-SEQUENCE ANALYSIS OF GENES ENCODING NONTYPABLE HAEMOPHILUS-INFLUENZAE HIGH-MOLECULAR-WEIGHT SURFACE-EXPOSED PROTEINS RELATED TO FILAMENTOUS HEMAGGLUTININ OF BORDETELLA-PERTUSSIS [J].

BARENKAMP, SJ ;

LEININGER, E .

INFECTION AND IMMUNITY, 1992, 60 (04) :1302-1313

[4] SEPA, THE MAJOR EXTRACELLULAR PROTEIN OF SHIGELLA-FLEXNERI - AUTONOMOUS SECRETION AND INVOLVEMENT IN TISSUE INVASION [J].

BENJELLOUNTOUIMI, Z ;

SANSONETTI, PJ ;

PARSOT, C .

MOLECULAR MICROBIOLOGY, 1995, 17 (01) :123-135

[5] AIDA-I, THE ADHESIN INVOLVED IN DIFFUSE ADHERENCE OF THE DIARRHEOGENIC ESCHERICHIA-COLI STRAIN-2787 (O126-H27), IS SYNTHESIZED VIA A PRECURSOR MOLECULE [J].

BENZ, I ;

SCHMIDT, MA .

MOLECULAR MICROBIOLOGY, 1992, 6 (11) :1539-1546

[6] ASYMPTOMATIC CARRIAGE OF NEISSERIA-MENINGITIDIS IN A RANDOMLY SAMPLED POPULATION [J].

CAUGANT, DA ;

HOIBY, EA ;

MAGNUS, P ;

SCHEEL, O ;

HOEL, T ;

BJUNE, G ;

WEDEGE, E ;

ENG, J ;

FROHOLM, LO .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (02) :323-330

[7] COMPARISON OF METHODS FOR THE ANALYSIS OF OUTER-MEMBRANE ANTIGENS OF NEISSERIA-MENINGITIDIS BY WESTERN BLOTTING [J].

DAVIES, RL ;

WALL, RA ;

BORRIELLO, SP .

JOURNAL OF IMMUNOLOGICAL METHODS, 1990, 134 (02) :215-225

[8]

GRUBER A, 1995, BIOTECHNIQUES, V19, P28

[9] The great escape: structure and function of the autotransporter proteins [J].

Henderson, IR ;

Navarro-Garcia, F ;

Nataro, JP .

TRENDS IN MICROBIOLOGY, 1998, 6 (09) :370-378

[10] CONJUGATES OF SYNTHETIC CYCLIC-PEPTIDES ELICIT BACTERICIDAL ANTIBODIES AGAINST A CONFORMATIONAL EPITOPE ON A CLASS-1 OUTER-MEMBRANE PROTEIN OF NEISSERIA-MENINGITIDIS [J].

HOOGERHOUT, P ;

DONDERS, EMLM ;

VANGAANSVANDENBRINK, JAM ;

KUIPERS, B ;

BRUGGHE, HF ;

VANUNEN, LMA ;

TIMMERMANS, HAM ;

TENHOVE, GJ ;

DEJONG, APJM ;

WIERTZ, EJHJ ;

POOLMAN, JT .

INFECTION AND IMMUNITY, 1995, 63 (09) :3473-3478