Genetic evidence for CheB- and CheR-dependent chemotaxis system in A. tumefaciens toward acetosyringone

被引:19
作者
Harighi, Behrouz [1 ]
机构
[1] Univ Kurdistan, Coll Agr, Dept Plant Protect, Sanandaj, Iran
关键词
Agrobacterium; Chemotaxis; Methylesterase; Methyltransferase; Acetosyringone; AGROBACTERIUM-TUMEFACIENS; BINDING-PROTEIN; VIRA PROTEIN; PLASMID; PHOSPHORYLATION; METHYLATION; VIRULENCE; GENOME; C58;
D O I
10.1016/j.micres.2008.11.001
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Agrobacterium tumefaciens has homologues of chemotaxis genes of the a-subgroup of proteobacteria, which include orf1, orf2, cheY1, cheA, cheR, cheB, cheY2, orf9, and orf10 and organized in one operon [Wright et at. A chemotaxis cluster from Agrobacterium tumefaciens. Gene 1998;220:83-9]. Two mutants of A. tumefaciens were created by in-frame deletions of cheB and cheR using suicide vector, pK18mobsacB. The chemosensory behaviour of mutants studied on swarm plates and in blind-well chemotaxis assay toward acetosyringone. Under the conditions tested, both CheR and CheB were essential for normal chemotaxis. (C) 2008 Elsevier GmbH. All rights reserved.
引用
收藏
页码:634 / 641
页数:8
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