Effects of a magnetic field and magnetization force on protein crystal growth. Why does a magnet improve the quality of some crystals?

被引:41
作者
Ataka, M
Wakayama, NI
机构
[1] Natl Inst Adv Ind Sci & Technol AIST, Ikeda 5638577, Japan
[2] Natl Inst Adv Ind Sci & Technol AIST, Tsukuba, Ibaraki 3058565, Japan
[3] Japan Sci & Technol Corp, CREST, Kawaguchi 3320012, Japan
来源
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷
关键词
D O I
10.1107/S0907444902014415
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Probable reasons why some protein crystals grown in a magnet exhibited better quality than control are discussed as follows. (1) Sedimenting three-dimensional nuclei are able to have the same orientation as the underlying, mother crystal into which the nuclei merge. (2) Protein solution may become more viscous, leading to reduction of convection. (3) If an upward force is generated by use of an inhomogeneous magnetic field, the effects of the density differences can be made less significant, causing the reduction of natural convection and the retardation of crystal sedimentation.
引用
收藏
页码:1708 / 1710
页数:3
相关论文
共 25 条
[1]   Protein crystals orientation in a magnetic field [J].
Astier, JP ;
Veesler, S ;
Boistelle, R .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :703-706
[2]   α-amylase crystal growth investigated by in situ atomic force microscopy [J].
Astier, JP ;
Bokern, D ;
Lapena, L ;
Veesler, S .
JOURNAL OF CRYSTAL GROWTH, 2001, 226 (2-3) :294-302
[3]   Magnetic orientation as a tool to study the initial stage of crystallization of lysozyme [J].
Ataka, M ;
Katoh, E ;
Wakayama, NI .
JOURNAL OF CRYSTAL GROWTH, 1997, 173 (3-4) :592-596
[4]   Nucleation in protein crystallization [J].
Drenth, J ;
Haas, C .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :867-872
[5]   Understanding protein crystallization on the basis of the phase diagram [J].
Haas, C ;
Drenth, J .
JOURNAL OF CRYSTAL GROWTH, 1999, 196 (2-4) :388-394
[6]   Apparent viscosity of human blood in a high static magnetic field [J].
Haik, Y ;
Pai, VN ;
Chen, CJ .
JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS, 2001, 225 (1-2) :180-186
[7]   Magnet used for protein crystallization: Novel attempts to improve the crystal quality [J].
Lin, SX ;
Zhou, M ;
Azzi, A ;
Xu, GJ ;
Wakayama, NI ;
Ataka, M .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2000, 275 (02) :274-278
[8]  
Malkin AJ, 1996, PROTEINS, V24, P247, DOI 10.1002/(SICI)1097-0134(199602)24:2<247::AID-PROT11>3.0.CO
[9]  
2-N
[10]   MECHANISMS OF GROWTH FOR PROTEIN AND VIRUS CRYSTALS [J].
MALKIN, AJ ;
KUZNETSOV, YG ;
LAND, TA ;
DEYOREO, JJ ;
MCPHERSON, A .
NATURE STRUCTURAL BIOLOGY, 1995, 2 (11) :956-959