Leaderless mRNAs bind 70S ribosomes more strongly than 30S ribosomal subunits in Escherichia coli

被引：54

作者：

O'Donnell, SA ^{[1
]}

Janssen, GR ^{[1
]}

机构：

[1] Miami Univ, Dept Microbiol, Oxford, OH 45056 USA

来源：

JOURNAL OF BACTERIOLOGY | 2002年 / 184卷 / 23期

关键词：

D O I：

10.1128/JB.184.23.6730-6733.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

By primer extension inhibition assays, 70S ribosomes bound with higher affinity, or stability, than did 30S subunits to leaderless mRNAs containing AUG or GUG start codons. Addition of translation initiation factors affected ribosome binding to leaderless mRNAs. Our results suggest that translation of leaderless mRNAs might initiate through a pathway involving 70S ribosomes or 30S subunits lacking IF3.

引用

收藏

页码：6730 / 6733

页数：4

相关论文

共 26 条

[1] Effect of alkylammonium intercalated montmorillonite as filler on natural rubber [J].

Bala, P ;

Samantaray, BK ;

Srivastava, SK ;

Nando, GB .

JOURNAL OF MATERIALS SCIENCE LETTERS, 2001, 20 (06) :563-564

[2] Crystal structure of an initiation factor bound to the 30S ribosomal subunit [J].

Carter, AP ;

Clemons, WM ;

Brodersen, DE ;

Morgan-Warren, RJ ;

Hartsch, T ;

Wimberly, BT ;

Ramakrishnan, V .

SCIENCE, 2001, 291 (5503) :498-501

[3] BACTERIOPHAGE-P2 LATE PROMOTERS .2. COMPARISON OF THE 4 LATE PROMOTER SEQUENCES [J].

CHRISTIE, GE ;

CALENDAR, R .

JOURNAL OF MOLECULAR BIOLOGY, 1985, 181 (03) :373-382

[4] Selective stimulation of translation of leaderless mRNA by initiation factor 2:: evolutionary implications for translation [J].

Grill, S ;

Gualerzi, CO ;

Londei, P ;

Bläsi, U .

EMBO JOURNAL, 2000, 19 (15) :4101-4110

[5] Modulation of ribosomal recruitment to 5′-terminal start codons by translation initiation factors IF2 and IF3 [J].

Grill, S ;

Moll, I ;

Hasenöhrl, D ;

Gualerzi, CO ;

Bläsi, U .

FEBS LETTERS, 2001, 495 (03) :167-171

[6] INITIATION OF MESSENGER-RNA TRANSLATION IN PROKARYOTES [J].

GUALERZI, CO ;

PON, CL .

BIOCHEMISTRY, 1990, 29 (25) :5881-5889

[7]

HARTZ D, 1988, METHOD ENZYMOL, V164, P419

[8] SPECIALIZED RIBOSOME SYSTEM - PREFERENTIAL TRANSLATION OF A SINGLE MESSENGER-RNA SPECIES BY A SUBPOPULATION OF MUTATED RIBOSOMES IN ESCHERICHIA-COLI [J].

HUI, A ;

DEBOER, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4762-4766

[9] A SINGLE BASE CHANGE IN THE SHINE-DALGARNO REGION OF 16S RIBOSOMAL-RNA OF ESCHERICHIA-COLI AFFECTS TRANSLATION OF MANY PROTEINS [J].

JACOB, WF ;

SANTER, M ;

DAHLBERG, AE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (14) :4757-4761

[10] EXPRESSION, PURIFICATION AND OPERATOR BINDING OF THE TRANSPOSON TN1721-ENCODED TET REPRESSOR [J].

KLOCK, G ;

HILLEN, W .

JOURNAL OF MOLECULAR BIOLOGY, 1986, 189 (04) :633-641