Immunological Reactivity of Blood from Healthy Humans to the rAls3p-N Vaccine Protein

被引：16

作者：

Baquir, Beverlie ^{[1
]}

Lin, Lin ^{[1
]}

Ibrahim, Ashraf S. ^{[1
,3
]}

Fu, Yue ^{[1
,3
]}

Avanesian, Valentina ^{[1
]}

Tu, Ang ^{[1
]}

Edwards, John, Jr. ^{[1
,3
]}

Spellberg, Brad ^{[1
,2
,3
]}

机构：

[1] Harbor Univ Calif Los Angeles, Med Ctr, Los Angeles Biomed Res Inst, Div Infect Dis, Torrance, CA USA

[2] Harbor Univ Calif Los Angeles, Med Ctr, Div Gen Internal Med, Torrance, CA USA

[3] Univ Calif Los Angeles, David Geffen Sch Med, Los Angeles, CA 90095 USA

来源：

JOURNAL OF INFECTIOUS DISEASES | 2010年 / 201卷 / 03期

关键词：

STREPTOCOCCUS-PNEUMONIAE; ANTIBODY; EPIDEMIOLOGY; INFECTIONS; PROTECTION; CHILDREN; INFANTS; AGE;

D O I：

10.1086/649901

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We determined reactivity of human blood to a vaccine based on the recombinant N-terminus of candidal Als3p (rAls3p-N) in preparation for future clinical trials. Healthy donor plasma had high immunoglobulin Gtiters (median, 1:51,200) and lower immunoglobulin A (median, 1:3,200) and immunoglobulin E (median, 1:128) titers to rAls3p-N by enzyme-linked immunosorbent assay. rAls3p-N stimulated interferon gamma (IFN-gamma) and interleukin (IL)-17, but not IL-4, from donor lymphocytes by enzyme-linked immunosorbent spot assay and IL-12 p70, IFN-gamma, IL-17, and IL-10 by cytometric bead array. Donors reacted to diverse immunodominant epitopes. Thus, facile humoral and cellular assays can monitor immune responses to the rAls3p-N vaccine in planned clinical trials.

引用

页码：473 / 477

页数：5

共 17 条

[1] A RANDOMIZED, PROSPECTIVE FIELD TRIAL OF A CONJUGATE VACCINE IN THE PROTECTION OF INFANTS AND YOUNG-CHILDREN AGAINST INVASIVE HAEMOPHILUS-INFLUENZAE TYPE-B DISEASE [J].