Transcription from sigma(54)-dependent bacterial promoters can be regarded as a second paradigm for bacterial gene transcription. The initial sigma(54)-RNA polymerase (RNAP)center dot promoter complex, the closed complex, is transcriptionally silent. The transcriptionally proficient sigma(54)-RNAP center dot promoter complex, the open complex, is formed upon remodeling of the closed complex by actions of a specialized activator protein that belongs to the AAA ((A) under bar TPases (a) under bar ssociated with various cellular (a) under bar ctivities) protein family in an ATP hydrolysis-dependent reaction. The integrity of a highly conserved signature motif in the AAA activator (known as the GAFTGA motif) is important for the remodeling activity of the AAA activator and for open complex formation. We now provide evidence that the invariant threonine residue of the GAFTGA motif plays a role in sensing the DNA downstream of the sigma(54)-RNAP-binding site and in coupling this information to sigma(54)-RNAP via the conserved regulatory Region I domain of sigma(54) during open complex formation.
机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
De Carlo, Sacha
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Chen, Baoyu
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Chen, Baoyu
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Hoover, Timothy R.
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Hoover, Timothy R.
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Kondrashkina, Elena
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Kondrashkina, Elena
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Nogales, Eva
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Nogales, Eva
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Nixon, B. Tracy
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Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
De Carlo, Sacha
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Chen, Baoyu
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Chen, Baoyu
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Hoover, Timothy R.
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Hoover, Timothy R.
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Kondrashkina, Elena
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Kondrashkina, Elena
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Nogales, Eva
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机构:Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
Nogales, Eva
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Nixon, B. Tracy
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Univ Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USAUniv Calif Berkeley, Howard Hughes Med Inst, Dept Mol & Cell Biol, Berkeley, CA 94720 USA