Prevalence of genotypes of Orientia tsutsugamushi in patients with scrub typhus in Miyazaki prefecture

被引：5

作者：

Horinouchi, H

Murai, K

Okayama, A

Nagatomo, Y

Tachibana, N

Tsubouchi, H

机构：

[1] MIYAZAKI UNIV,SCH MED,DEPT INTERNAL MED 2,MIYAZAKI 88916,JAPAN

[2] OITA PREFECTURAL INST HLTH & ENVIRONM,OITA 870,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 41卷 / 06期

关键词：

Orientia tsutsugamushi; genotype;

D O I：

10.1111/j.1348-0421.1997.tb01884.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The genotypes of Orientia tsutsugamushi in patients with scrub typhus in Miyazaki Prefecture were examined by polymerase chain reaction (PCR) and the restriction fragment length polymorphism method. Specific patterns for genotypes Irie, Hirano, Tazume and Yoshimura were detected in 26, 6, 5 and 2 of 39 DNA samples obtained from peripheral blood mononuclear cells, respectively. DNA sequences of the PCR products from the Tazume strain were genetically very close to the Hirano strain and the Yoshimura strain was also very close to the Karp strain, Furthermore, the DNA sequences from the Irie and Tazume strains were completely homologous to the reported sequences of the Kawasaki and Kuroki strains, respectively.

引用

收藏

页码：503 / 507

页数：5

相关论文

共 15 条

[1] SEROTYPE-SPECIFIC AMPLIFICATION OF RICKETTSIA-TSUTSUGAMUSHI DNA BY NESTED POLYMERASE CHAIN-REACTION [J].

FURUYA, Y ;

YOSHIDA, Y ;

KATAYAMA, T ;

YAMAMOTO, S ;

KAWAMURA, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (06) :1637-1640

[2] Genotypic identification of Rickettsia tsutsugamushi by restriction fragment length polymorphism analysis of DNA amplified by the polymerase chain reaction [J].

Horinouchi, H ;

Murai, K ;

Okayama, A ;

Nagatomo, Y ;

Tachibana, N ;

Tsubouchi, H .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1996, 54 (06) :647-651

[3]

KAIHO I, 1993, J JPN ASS INFECT DIS, V67, P196

[4] EPIDEMIOLOGY OF TSUTSUGAMUSHI DISEASE IN RELATION TO THE SEROTYPES OF RICKETTSIA-TSUTSUGAMUSHI ISOLATED FROM PATIENTS, FIELD MICE, AND UNFED CHIGGERS ON THE EASTERN SLOPE OF MOUNT FUJI, SHIZUOKA PREFECTURE, JAPAN [J].

KAWAMORI, F ;

AKIYAMA, M ;

SUGIEDA, M ;

KANDA, T ;

AKAHANE, S ;

UCHIKAWA, K ;

YAMADA, Y ;

KUMADA, N ;

FURUYA, Y ;

YOSHIDA, Y ;

YAMAMOTO, S ;

OHASHI, N ;

TAMURA, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (11) :2842-2846

[5] 2-STEP POLYMERASE CHAIN-REACTION FOR DIAGNOSIS OF SCRUB TYPHUS AND IDENTIFICATION OF ANTIGENIC VARIANTS OF RICKETTSIA-TSUTSUGAMUSHI [J].

KAWAMORI, F ;

AKIYAMA, M ;

SUGIEDA, M ;

KANDA, T ;

AKAHANE, S ;

YAMAMOTO, S ;

OHASHI, N ;

TAMURA, A .

JOURNAL OF VETERINARY MEDICAL SCIENCE, 1993, 55 (05) :749-755

[6] HIGH-LEVEL EXPRESSION OF A 56-KILODALTON PROTEIN GENE (BOR56) OF RICKETTSIA-TSUTSUGAMUSHI BORYONG AND ITS APPLICATION TO ENZYME-LINKED IMMUNOSORBENT ASSAYS [J].

KIM, IS ;

SEONG, SY ;

WOO, SG ;

CHOI, MS ;

CHANG, WH .

JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (03) :598-605

[7] SENSITIVITY OF POLYMERASE CHAIN-REACTION ASSAY FOR RICKETTSIA-TSUTSUGAMUSHI IN PATIENTS BLOOD-SAMPLES [J].

MURAI, K ;

TACHIBANA, N ;

OKAYAMA, A ;

SHISHIME, E ;

TSUDA, K ;

OSHIKAWA, T .

MICROBIOLOGY AND IMMUNOLOGY, 1992, 36 (11) :1145-1153

[8] CLONING AND SEQUENCING OF THE GENE (TSG56) ENCODING A TYPE-SPECIFIC ANTIGEN FROM RICKETTSIA-TSUTSUGAMUSHI [J].

OHASHI, N ;

NASHIMOTO, H ;

IKEDA, H ;

TAMURA, A .

GENE, 1990, 91 (01) :119-122

[9]

OHASHI N, 1992, J BIOL CHEM, V267, P12728

[10] THE 56-KILODALTON MAJOR PROTEIN ANTIGEN OF RICKETTSIA-TSUTSUGAMUSHI - MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE STA56 GENE AND PRECISE IDENTIFICATION OF A STRAIN-SPECIFIC EPITOPE [J].

STOVER, CK ;

MARANA, DP ;

CARTER, JM ;

ROE, BA ;

MARDIS, E ;

OAKS, EV .

INFECTION AND IMMUNITY, 1990, 58 (07) :2076-2084