Rapid quantification of bacterial cells in potable water using a simplified microfluidic device

被引：29

作者：

Sakamoto, Chieko

Yamaguchi, Nobuyasu

Yamada, Masumi

Nagase, Hiroyasu

Seki, Minoru

Nasu, Masao

机构：

[1] Osaka Univ, Grad Sch Pharmaceut Sci, Suita, Osaka 5650871, Japan

[2] Univ Tokyo, Sch Engn, Bunkyo Ku, Tokyo 1138656, Japan

[3] Osaka Prefecture Univ, Grad Sch Engn, Sakai, Osaka 5998531, Japan

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2007年 / 68卷 / 03期

关键词：

microfluidic device; potable water; total direct count;

D O I：

10.1016/j.mimet.2006.11.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified microfluidic device for quantification of bacteria in potable water was fabricated and examined. Comparisons of counts of Escherichia coli by the microfluidic system and by epifluorescence microscopy closely correlated (r(2)=0.99). Bacteria in natural mineral water and in purified household tap water were accurately enumerated by using this system within 15 min after fluorescent staining. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：643 / 647

页数：5

共 30 条

[1] Modular concept of a laboratory on a chip for chemical and biochemical analysis [J].