An integrated microfabricated cell sorter

被引：422

作者：

Fu, AY

Chou, HP

Spence, C

Arnold, FH

Quake, SR

机构：

[1] CALTECH, Dept Appl Phys, Pasadena, CA 91125 USA

[2] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

ANALYTICAL CHEMISTRY | 2002年 / 74卷 / 11期

关键词：

D O I：

10.1021/ac0255330

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We have developed an integrated microfabricated cell sorter using multilayer soft lithography. This integrated cell sorter is incorporated with various microfluidic functionalities, including peristaltic pumps, dampers, switch valves, and input and output wells, to perform cell sorting in a coordinated and automated fashion. The active volume of an actuated valve on this integrated cell sorter can be as small as 1 pL, and the volume of optical interrogation is similar to100 fL. Different algorithms of cell manipulation, including cell trapping, were implemented in these devices. We have also demonstrated sorting and recovery of Escherichia coli cells on the chip.

引用

页码：2451 / 2457

页数：7

共 25 条

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