Human Platelet Lysate versus Fetal Calf Serum: These Supplements Do Not Select for Different Mesenchymal Stromal Cells

被引：57

作者：

Fernandez-Rebollo, Eduardo ^{[1
,2
]}

Mentrup, Birgit ^{[3
]}

Ebert, Regina ^{[3
]}

Franzen, Julia ^{[1
,2
]}

Abagnale, Giulio ^{[1
,2
]}

Sieben, Torsten ^{[1
,2
]}

Ostrowska, Alina ^{[1
,2
]}

Hoffmann, Per ^{[4
,5
]}

Roux, Pierre-Francois ^{[6
]}

Rath, Bjoern ^{[7
]}

Goodhardt, Michele ^{[8
]}

Lemaitre, Jean-Marc ^{[9
]}

Bischof, Oliver ^{[6
]}

Jakob, Franz ^{[3
]}

Wagner, Wolfgang ^{[1
,2
]}

机构：

[1] Rhein Westfal TH Aachen, Sch Med, Helmholtz Inst Biomed Engn Stem Cell Biol & Cell, D-52074 Aachen, Germany

[2] Rhein Westfal TH Aachen, Sch Med, Inst Biomed Technol Cell Biol, D-52074 Aachen, Germany

[3] Univ Wurzburg, Orthoped Ctr Musculoskeletal Res, Dept Orthoped, D-97074 Wurzburg, Germany

[4] Univ Bonn, Dept Genom, Inst Human Genet, D-53127 Bonn, Germany

[5] Univ Basel, Dept Biomed, Human Genom Res Grp, CH-4031 Basel, Switzerland

[6] Inst Pasteur, INSERM, U993, Lab Nucl Org & Oncogenesis,Dept Cell Biol & Infec, F-75015 Paris, France

[7] Rhein Westfal TH Aachen, Sch Med, Dept Orthoped, D-52074 Aachen, Germany

[8] Univ Paris Diderot, INSERM, UMRS 1126, Inst Univ Hematol, F-75010 Paris, France

[9] Univ Montpellier, INSERM, U1183, IRMB, F-34295 Montpellier 05, France

来源：

SCIENTIFIC REPORTS | 2017年 / 7卷

关键词：

STEM-CELLS; BOVINE SERUM; ANIMAL SERUM; EXPANSION; CULTURE; HETEROGENEITY; ALTERNATIVES; SUBSTITUTE; GROWTH;

D O I：

10.1038/s41598-017-05207-1

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Culture medium of mesenchymal stromal cells (MSCs) is usually supplemented with either human platelet lysate (HPL) or fetal calf serum (FCS). Many studies have demonstrated that proliferation and cellular morphology are affected by these supplements - it is therefore important to determine if they favor outgrowth of different subpopulations and thereby impact on the heterogeneous composition of MSCs. We have isolated and expanded human bone marrow-derived MSCs in parallel with HPL or FCS and demonstrated that HPL significantly increases proliferation and leads to dramatic differences in cellular morphology. Remarkably, global DNA-methylation profiles did not reveal any significant differences. Even at the transcriptomic level, there were only moderate changes in pairwise comparison. Furthermore, the effects on proliferation, cytoskeletal organization, and focal adhesions were reversible by interchanging to opposite culture conditions. These results indicate that cultivation of MSCs with HPL or FCS has no systematic bias for specific cell types.

引用

页数：8

共 29 条

[1]

Surface topography enhances differentiation of mesenchymal stem cells towards osteogenic and adipogenic lineages [J].