BoneMA-synthesis and characterization of a methacrylated bone-derived hydrogel for bioprinting of in-vitro vascularized tissue constructs

被引:33
作者
Parthiban, S. Prakash [1 ]
Athirasala, Avathamsa [2 ]
Tahayeri, Anthony [1 ]
Abdelmoniem, Reyan [1 ]
George, Anne [3 ]
Bertassoni, Luiz E. [1 ,2 ,4 ,5 ]
机构
[1] Oregon Hlth & Sci Univ, Sch Dent, Dept Restorat Dent, Div Biomat & Biomech, Portland, OR 97201 USA
[2] Oregon Hlth & Sci Univ, Dept Biomed Engn, Collaborat Life Sci Bldg, Portland, OR 97201 USA
[3] Univ Illinois, Dept Oral Biol, Chicago, IL USA
[4] Oregon Hlth & Sci Univ, Ctr Regenerat Med, Portland, OR 97201 USA
[5] Oregon Hlth & Sci Univ, Knight Canc Inst, Canc Early Detect Adv Res CEDAR Ctr, Portland, OR 97201 USA
关键词
extracellular matrix; hydrogels; human bone grafts; granular microgels; bioinks; bioprinting; vascularization;
D O I
10.1088/1758-5090/abb11f
中图分类号
R318 [生物医学工程];
学科分类号
100103 [病原生物学];
摘要
It has long been proposed that recapitulating the extracellular matrix (ECM) of native human tissues in the laboratory may enhance the regenerative capacity of engineered scaffolds in-vivo. Organ- and tissue-derived decellularized ECM biomaterials have been widely used for tissue repair, especially due to their intrinsic biochemical cues that can facilitate repair and regeneration. The main purpose of this study was to synthesize a new photocrosslinkable human bone-derived ECM hydrogel for bioprinting of vascularized scaffolds. To that end, we demineralized and decellularized human bone fragments to obtain a bone matrix, which was further processed and functionalized with methacrylate groups to form a photocrosslinkable methacrylate bone ECM hydrogel- bone-derived biomaterial (BoneMA). The mechanical properties of BoneMA were tunable, with the elastic modulus increasing as a function of photocrosslinking time, while still retaining the nanoscale features of the polymer networks. The intrinsic cell-compatibility of the bone matrix ensured the synthesis of a highly cytocompatible hydrogel. The bioprinted BoneMA scaffolds supported vascularization of endothelial cells and within a day led to the formation of interconnected vascular networks. We propose that such a quick vascular network formation was due to the host of pro-angiogenic biomolecules present in the bone ECM matrix. Further, we also demonstrate the bioprintability of BoneMA in microdimensions as injectable ECM-based building blocks for microscale tissue engineering in a minimally invasive manner. We conclude that BoneMA may be a useful hydrogel system for tissue engineering and regenerative medicine.
引用
收藏
页数:13
相关论文
共 53 条
[1]
Bone extracellular matrix hydrogel enhances osteogenic differentiation of C2C12 myoblasts and mouse primary calvarial cells [J].
Alom, Noura ;
Peto, Heather ;
Kirkham, Glen R. ;
Shakesheff, Kevin M. ;
White, Lisa J. .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2018, 106 (02) :900-908
[2]
Engineered cell-laden human protein-based elastomer [J].
Annabi, Nasim ;
Mithieux, Suzanne M. ;
Zorlutuna, Pinar ;
Camci-Unal, Gulden ;
Weiss, Anthony S. ;
Khademhosseini, Ali .
BIOMATERIALS, 2013, 34 (22) :5496-5505
[3]
Bioinks and bioprinting technologies to make heterogeneous and biomimetic tissue constructs [J].
Ashammakhi, N. ;
Ahadian, S. ;
Xu, C. ;
Montazerian, H. ;
Ko, H. ;
Nasiri, R. ;
Barros, N. ;
Khademhosseini, A. .
MATERIALS TODAY BIO, 2019, 1
[4]
Vitamin A Deficiency and Alterations in the Extracellular Matrix [J].
Barber, Teresa ;
Esteban-Pretel, Guillermo ;
Pilar Marin, Maria ;
Timoneda, Joaquin .
NUTRIENTS, 2014, 6 (11) :4984-5017
[5]
Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs [J].
Bertassoni, Luiz E. ;
Cecconi, Martina ;
Manoharan, Vijayan ;
Nikkhah, Mehdi ;
Hjortnaes, Jesper ;
Cristino, Ana Luiza ;
Barabaschi, Giada ;
Demarchi, Danilo ;
Dokmeci, Mehmet R. ;
Yang, Yunzhi ;
Khademhosseini, Ali .
LAB ON A CHIP, 2014, 14 (13) :2202-2211
[6]
Photo-cross-linking of type I collagen gels in the presence of smooth muscle cells: Mechanical properties, cell viability, and function [J].
Brinkman, WT ;
Nagapudi, K ;
Thomas, BS ;
Chaikof, EL .
BIOMACROMOLECULES, 2003, 4 (04) :890-895
[7]
Controlled degradation and mechanical behavior of photopolymerized hyaluronic acid networks [J].
Burdick, JA ;
Chung, C ;
Jia, XQ ;
Randolph, MA ;
Langer, R .
BIOMACROMOLECULES, 2005, 6 (01) :386-391
[8]
Innovative encapsulation platform based on pancreatic extracellular matrix achieve substantial insulin delivery [J].
Chaimov, D. ;
Baruch, L. ;
Krishtul, S. ;
Meivar-Levy, I. ;
Ferber, S. ;
Machluf, M. .
JOURNAL OF CONTROLLED RELEASE, 2017, 257 :91-101
[9]
Injectable Supramolecular Hydrogel/Microgel Composites for Therapeutic Delivery [J].
Chen, Minna H. ;
Chung, Jennifer J. ;
Mealy, Joshua E. ;
Zaman, Samir ;
Li, Elizabeth C. ;
Arisi, Maria F. ;
Atluri, Pavan ;
Burdick, Jason A. .
MACROMOLECULAR BIOSCIENCE, 2019, 19 (01)
[10]
Chen XF, 2010, TISSUE ENG PT A, V16, P585, DOI 10.1089/ten.TEA.2009.0491