Cardiolipin is associated with the terminal oxidase of an extremely halophilic archaeon

被引：14

作者：

Corcelli, Angela ^{[1
]}

Lobasso, Simona ^{[1
]}

Palese, Luigi L. ^{[1
]}

Saponetti, Matilde Sublimi ^{[1
]}

Papa, Sergio ^{[1
]}

机构：

[1] Univ Bari, Dept Biochem Med, Bari, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 354卷 / 03期

关键词：

archaea; cytochrome c oxidase; lipid domain; bisphosphatidylglycerol; Halorubrum;

D O I：

10.1016/j.bbrc.2007.01.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Membranes having an a high content of cardiolipin were isolated from an extremely halophilic archaeon Halorubrum sp. Absorbance difference spectra of detergent-solubilized plasma membranes reduced by dithionite suggested the presence of b-type cytochromes. Non-denaturing gel electrophoresis revealed only one fraction having TMPD-oxidase activity in which cardiolipin was the major lipid component. The electroeluted fraction showed a cytochrome c oxidase activity characterized by the reduced minus oxidized difference spectra as a terminal heme-copper oxidase. The cytochrome c oxidase activity of the archaeal cardiolipin-rich membranes was inhibited by the cardiolipin-specific fluorescent marker 10-N-nonyl acridine orange (NAO) in a dose-dependent manner. The results indicate that ail archaeal analogue of cardiolipin is tightly associated to archaeal terminal oxidases and is required for its optimal functioning. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：795 / 801

页数：7

共 29 条

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