Expression profiling in mouse fetal thymus reveals clusters of coordinately expressed genes that mark individual stages of T-cell ontogeny

被引:4
作者
Ramialison, M
Mohr, E
Nal, B
Saboul, T
Carrier, A
Tagett, R
Granjeaud, S
Nguyen, C
Gautheret, D
Jordan, BR
Ferrier, P [1 ]
机构
[1] Univ Mediterranee, CNRS, INSERM, Ctr Immunol Marseille Luminy, F-13288 Marseille, France
[2] Inst Paoli Calmettes, F-13273 Marseille, France
[3] Marseille Genopole, F-13288 Marseille, France
[4] CIML, F-13288 Marseille 9, France
关键词
gene; genetics; DNA arrays; expression profiling; T-cell ontogeny;
D O I
10.1007/s00251-002-0488-y
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
To search for genes that participate in regulatory networks sustaining mouse embryonic T-cell development, we have performed expression profiling using nylon macroarrays. Labeled samples representative of individual developmental stages were utilized, taking advantage of cell homogeneity during early thymus ontogeny. cDNAs revealing differential expression were further selected using labeled samples derived from lymphoid versus non-lymphoid tissues, and from mutant thymi exhibiting T-cell developmental defects. We thus identified clusters of coexpressed genes during T-cell embryogenesis and characterized their sequences through bioinformatics. We compare our results with those from other profiling analyses in the immune system, and discuss their implications for the definition of genes whose products are involved in T-cell development.
引用
收藏
页码:469 / 478
页数:10
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