Movement of retinal along the visual transduction path

被引：205

作者：

Borhan, B

Souto, ML

Imai, H

Shichida, Y

Nakanishi, K ^{[1
]}

机构：

[1] Columbia Univ, Dept Chem, New York, NY 10027 USA

[2] Kyoto Univ, Grad Sch Sci, Dept Biophys, Kyoto 6068502, Japan

来源：

SCIENCE | 2000年 / 288卷 / 5474期

关键词：

D O I：

10.1126/science.288.5474.2209

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Movement of the ligand/receptor complex in rhodopsin (Rh) has been traced. Bleaching of diazoketo rhodopsin (DK-Rh) containing 11-cis-3-diazo-4-oxo-retinal yields batho-, lumi-, meta-I-, and meta-II-Rh intermediates corresponding to those of native kh but at lower temperatures. Photoaffinity labeling of DK-Rh and these bleaching intermediates shows that the ionone ring cross-links to tryptophan-265 on helix F in DK-Rh and batho-Rh, and to alanine-169 on helix D in lumi-, meta-I-, and meta-II-Rh intermediates, It is likely that these movements involving a flip-over of the chromophoric ring trigger changes in cytoplasmic membrane loops resulting in heterotrimeric guanine nucleotide-binding protein (G protein) activation.

引用

页码：2209 / 2212

页数：4

共 39 条

[21]

NAKAYAMA TA, 1990, J BIOL CHEM, V265, P15762

[22] CIRCULAR-DICHROISM OF METAIODOPSIN-II AND ITS BINDING TO TRANSDUCIN - A COMPARATIVE-STUDY BETWEEN META-II INTERMEDIATES OF IODOPSIN AND RHODOPSIN [J].