Studies on ferulic acid esterase activity in fungal lipases and cutinases

被引：27

作者：

Andersen, A ^{[1
]}

Svendsen, A ^{[1
]}

Vind, J ^{[1
]}

Lassen, SF ^{[1
]}

Hjort, C ^{[1
]}

Borch, K ^{[1
]}

Patkar, SA ^{[1
]}

机构：

[1] Novozymes AS, Dept Prot Chem, DK-2880 Bagsvaerd, Denmark

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2002年 / 26卷 / 1-2期

关键词：

Aspergillus niger; Thermomyces lanuginosa lipase; xylan;

D O I：

10.1016/S0927-7765(01)00312-5

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

In this study we have tested a number of lipases, lipase variants and cutinases for ferulic acid esterase activity, using ferulic acid ethyl ester as substrate. It was shown that Thermomyces lanuginosa lipase (TLL), Candida antartica lipase A, Candida antartica lipase B, Rhizomucor miehei lipase and Fusarium oxysporum lipase have no significant ferulic acid esterase activity. Thirteen variants of TLL were constructed based on a model of Aspergillus niger ferulic acid esterase A (FAE-A). Activity assay using ferulic acid ethyl ester as substrate gave, for FAE-A, 112 U/mg = 112 mumol ferulic acid released per min per mg enzyme. Two of the variants of TLL had significant ferulic acid esterase activity, TLLv1 (7 U/mg) and TLLv10 (20 U/mg). Both these variants contain the mutation F113Y that seems to be essential for ferulic acid esterase activity. In addition to lipase activity, three cutinases showed ferulic acid esterase activity, Aspergillus oryzae cutinase (5 U/mg), Fusarium solani pisi cutinase (13 U/mg), Humicola insolence cutinase (20 U/mg). (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：47 / 55

页数：9

共 39 条

[1]

ABO M, Patent No. 200034450

[2]

Aliwan FO, 1999, J SCI FOOD AGR, V79, P457, DOI 10.1002/(SICI)1097-0010(19990301)79:3<457::AID-JSFA283>3.3.CO

[3]

2-7

[4]

Bartolome B, 1997, APPL ENVIRON MICROB, V63, P208

[5] Interfacial activation of triglyceride lipase from Thermomyces (Humicola) lanuginosa:: Kinetic parameters and a basis for control of the lid [J].

Berg, OG ;

Cajal, Y ;

Butterfoss, GL ;

Grey, RL ;

Alsina, MA ;

Yu, BZ ;

Jain, MK .

BIOCHEMISTRY, 1998, 37 (19) :6615-6627

[6] RHIZOMUCOR-MIEHEI TRIGLYCERIDE LIPASE IS SYNTHESIZED AS A PRECURSOR [J].

BOEL, E ;

HUGEJENSEN, B ;

CHRISTENSEN, M ;

THIM, L ;

FIIL, NP .

LIPIDS, 1988, 23 (07) :701-706

[7]

Boel E., 1995, Patent No. [EP305216 B1, 305216]

[8]

BOEL E, 1988, Patent No. [305216, 0305216]

[9] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE [J].

BRADY, L ;

BRZOZOWSKI, AM ;

DEREWENDA, ZS ;

DODSON, E ;

DODSON, G ;

TOLLEY, S ;

TURKENBURG, JP ;

CHRISTIANSEN, L ;

HUGEJENSEN, B ;

NORSKOV, L ;

THIM, L ;

MENGE, U .

NATURE, 1990, 343 (6260) :767-770

[10] A MODEL FOR INTERFACIAL ACTIVATION IN LIPASES FROM THE STRUCTURE OF A FUNGAL LIPASE-INHIBITOR COMPLEX [J].

BRZOZOWSKI, AM ;

DEREWENDA, U ;

DEREWENDA, ZS ;

DODSON, GG ;

LAWSON, DM ;

TURKENBURG, JP ;

BJORKLING, F ;

HUGEJENSEN, B ;

PATKAR, SA ;

THIM, L .

NATURE, 1991, 351 (6326) :491-494

← 1 2 3 4 →