The use of gaseous ammonia for the deprotection and cleavage steps during the solid-phase synthesis of oligonucleotides, and analogs

被引：9

作者：

Iyer, RP

Yu, D

Xie, J

Zhou, W

Agrawal, S

机构：

[1] Hybridon Inc., Cambridge, MA 02139

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1997年 / 7卷 / 11期

关键词：

D O I：

10.1016/S0960-894X(97)00236-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The use of NH3 gas under pressure offers an efficient alternative to hot aqueous ammonium hydroxide for the deprotection, and cleavage steps during the large-scale synthesis of oligonucleotides, and their analogs prepared using N-pent-4-enoyl (PNT) nucleoside phosphoramidites and H-phosphonates. (C) 1997 Elsevier Science Ltd.

引用

页码：1443 / 1448

页数：6

共 18 条

[1]

AGRAWAL S, 1996, ANTISENSE THERAPEUTI, P1

[2]

Agrawal Sudhir, 1995, Current Opinion in Biotechnology, V6, P12, DOI 10.1016/0958-1669(95)80003-4

[3] ADVANCES IN THE SYNTHESIS OF OLIGONUCLEOTIDES BY THE PHOSPHORAMIDITE APPROACH [J].