Cleavage of oligodeoxyribonucleotides from controlled-pore glass supports and their rapid deprotection by gaseous amines

被引：30

作者：

Boal, JH

Wilk, A

Harindranath, N

Max, EE

Kempe, T

Beaucage, SL

机构：

[1] US FDA,CTR BIOL EVALUAT & RES,DIV HEMATOL PROD,BETHESDA,MD 20892

[2] BARRSKOGEN INC,BOWIE,MD 20720

来源：

NUCLEIC ACIDS RESEARCH | 1996年 / 24卷 / 15期

关键词：

D O I：

10.1093/nar/24.15.3115

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method for the deprotection of oligodeoxyribonucleotides has been developed. Gaseous amines such as ammonia or methylamine were employed under pressure to achieve mild and rapid deprotection conditions. For example, oligodeoxyribonucleotides having a (tent-butyl)phenoxyacetyl group for the protection of the exocyclic amino function of cytosine, adenine and guanine were released from controlled-pore glass supports and fully deprotected by ammonia or methylamine under gas phase conditions, at room temperature, within 35 or 2 min respectively.

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页码：3115 / 3117

页数：3

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