FAST CLEAVAGE AND DEPROTECTION OF OLIGONUCLEOTIDES

被引：98

作者：

REDDY, MP

HANNA, NB

FAROOQUI, F

机构：

[1] Beckman Instruments Inc., Fullerton, CA 92634

来源：

TETRAHEDRON LETTERS | 1994年 / 35卷 / 25期

关键词：

D O I：

10.1016/S0040-4039(00)73341-7

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We have developed methylamine/ammonia as a fast cleavage and deprotection reagent which effects complete cleavage of oligonucleotides from the solid support in 5 min at room temperature and complete deprotection in 5 min at 65 degrees C. The problem of transamination side product formation, faced with the commonly used dC(bz) (10.0% side product) upon treatment with methylamine/ammonia, has been successfully solved by the use of dC(ac) (0.0% side product). DMT dC(ac) phosphoramidite-methylamine/ammonia system furnished oligonucleotides in equal or superior quality as compared to the other systems.

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页码：4311 / 4314

页数：4

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