A new method of defense response analysis using a transient expression system in rice protoplasts

被引：27

作者：

Takai, Ryota

Kaneda, Takashi

Isogai, Akira

Takayama, Seiji

Che, Fang-Sik

机构：

[1] Nagahama Inst Biosci & Technol, Fac Biosci, Dept Environm Biol, Shiga 5260829, Japan

[2] Nara Inst Sci & Technol, Grad Sch Biol Sci, NAIST, Nara 6300191, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2007年 / 71卷 / 02期

关键词：

protoplast; transient expression; rice; luciferase; defense response;

D O I：

10.1271/bbb.60526

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We established a new plant defense response assay using a transient expression system in rice protoplasts. The assay system sensitively detected defense induction by flagellin, which had previously been assigned to a specific elicitor. Our assay system provides a rapid and efficient way to dissect rice defense mechanisms.

引用

页码：590 / 593

页数：4

共 12 条

[1] A transient RNA interference assay system using Arabidopsis protoplasts [J].

An, CI ;

Sawada, A ;

Fukusaki, E ;

Kobayashi, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 2003, 67 (12) :2674-2677

[2]

BABA A, 1986, PLANT CELL PHYSIOL, V27, P463

[3] A novel system for gene silencing using siRNAs in rice leaf and stem-derived protoplasts [J].

Bart, Rebecca ;

Chern, Mawsheng ;

Park, Chang-Jin ;

Bartley, Laura ;

Ronald, Pamela C. .

PLANT METHODS, 2006, 2 (1)

[4] Flagellin from an incompatible strain of Pseudomonas avenae induces a resistance response in cultured rice cells [J].

Che, FS ;

Nakajima, Y ;

Tanaka, N ;

Iwano, M ;

Yoshida, T ;

Takayama, S ;

Kadota, I ;

Isogai, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (41) :32347-32356

[5] A highly efficient transient protoplast system for analyzing defence gene expression and protein-protein interactions in rice [J].

Chen, Songbiao ;

Tao, Lizen ;

Zeng, Lirong ;

Vega-Sanchez, Miguel E. ;

Umemura, Kenji ;

Wang, Guo-Liang .

MOLECULAR PLANT PATHOLOGY, 2006, 7 (05) :417-427

[6] Plants have a sensitive perception system for the most conserved domain of bacterial flagellin [J].

Felix, G ;

Duran, JD ;

Volko, S ;

Boller, T .

PLANT JOURNAL, 1999, 18 (03) :265-276

[7] Rice cDNA microatray-based gene expression profiling of the response to flagellin perception in cultured rice cells [J].

Fujiwara, S ;

Tanaka, N ;

Kaneda, T ;

Takayama, S ;

Isogai, A ;

Che, FS .

MOLECULAR PLANT-MICROBE INTERACTIONS, 2004, 17 (09) :986-998

[8] FLS2:: An LRR receptor-like kinase involved in the perception of the bacterial elicitor flagellin in Arabidopsis [J].

Gómez-Gómez, L ;

Boller, T .

MOLECULAR CELL, 2000, 5 (06) :1003-1011

[9] The map-based sequence of the rice genome [J].

Matsumoto, T ;

Wu, JZ ;

Kanamori, H ;

Katayose, Y ;

Fujisawa, M ;

Namiki, N ;

Mizuno, H ;

Yamamoto, K ;

Antonio, BA ;

Baba, T ;

Sakata, K ;

Nagamura, Y ;

Aoki, H ;

Arikawa, K ;

Arita, K ;

Bito, T ;

Chiden, Y ;

Fujitsuka, N ;

Fukunaka, R ;

Hamada, M ;

Harada, C ;

Hayashi, A ;

Hijishita, S ;

Honda, M ;

Hosokawa, S ;

Ichikawa, Y ;

Idonuma, A ;

Iijima, M ;

Ikeda, M ;

Ikeno, M ;

Ito, K ;

Ito, S ;

Ito, T ;

Ito, Y ;

Iwabuchi, A ;

Kamiya, K ;

Karasawa, W ;

Kurita, K ;

Katagiri, S ;

Kikuta, A ;

Kobayashi, H ;

Kobayashi, N ;

Machita, K ;

Maehara, T ;

Masukawa, M ;

Mizubayashi, T ;

Mukai, Y ;

Nagasaki, H ;

Nagata, Y .

NATURE, 2005, 436 (7052) :793-800

[10]

Sheen J, 2001, PLANT PHYSIOL, V127, P1466, DOI 10.1104/pp.010820

← 1 2 →