Novel membranes and surface modification able to activate specific cellular responses

被引：34

作者：

De Bartolo, Loredana

Morelli, Sabrina

Piscioneri, Antonella

Lopez, Linda C.

Favia, Pietro

d'Agostino, Riccardo

Drioli, Enrico

机构：

[1] Univ Calabria, Inst Membrane Technol, Natl Res Council Italy, I-87030 Arcavacata Di Rende, CS, Italy

[2] Univ Bari, Dept Chem, Bari, Italy

来源：

BIOMOLECULAR ENGINEERING | 2007年 / 24卷 / 01期

关键词：

membrane; human hepatocyte; liver specific functions; biotransformation; surface modification; MODIFIED POLYMERS; HUMAN HEPATOCYTES; PLASMA; ATTACHMENT; FIBRONECTIN; VIABILITY; ADHESION; CULTURE; CELLS;

D O I：

10.1016/j.bioeng.2006.07.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The design of new polymeric biomaterials together with new strategies to modify membrane surface are crucial to optimise cell-biomaterial interactions in vivo and in vitro biohybrid systems. In this study we report on the novel semipermeable membranes synthesised from a polymeric blend of modified polyetheretherketone and polyurethane able to support the long-term maintenance and differentiation of human liver cells and on the surface modification of polyethersulfone membranes by plasma polymerisation of acrylic acid monomers and by immobilization of arginine-glycine-aspartic acid (RGD) peptide through a hydrophilic "spacer arm" molecule. The performance of the modified and unmodified membranes was tested by evaluation of the liver function expression of primary human hepatocytes in terms of albumin production, protein secretion and drug biotransformation. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：23 / 26

页数：4

共 16 条

[1]

Altankov G, 2000, J BIOMED MATER RES, V52, P219, DOI 10.1002/1097-4636(200010)52:1<219::AID-JBM28>3.0.CO

[2]

2-F

[3] Plasma copolymer surfaces of acrylic acid 1,7 octadiene: Surface characterisation and the attachment of ROS 17/2.8 osteoblast-like cells [J].

Daw, R ;

Candan, S ;

Beck, AJ ;

Devlin, AJ ;

Brook, IM ;

MacNeil, S ;

Dawson, RA ;

Short, RD .

BIOMATERIALS, 1998, 19 (19) :1717-1725

[4] Effect of isoliquiritigenin on viability and differentiated functions of human hepatocytes maintained on PEEK-WC-polyurethane membranes [J].

De Bartolo, L ;

Morelli, S ;

Gallo, MC ;

Campana, C ;

Statti, G ;

Rende, M ;

Salerno, S ;

Drioli, E .

BIOMATERIALS, 2005, 26 (33) :6625-6634

[5] Biotransformation and liver-specific functions of human hepatocytes in culture on RGD-immobilized plasma-processed membranes [J].

De Bartolo, L ;

Morelli, S ;

Lopez, LC ;

Giorno, L ;

Campana, C ;

Salerno, S ;

Rende, M ;

Favia, P ;

Detomaso, L ;

Gristina, R ;

d'Agostino, R ;

Drioll, E .

BIOMATERIALS, 2005, 26 (21) :4432-4441

[6] A novel full-scale flat membrane bioreactor utilizing porcine hepatocytes: Cell viability and tissue-specific functions [J].

De Bartolo, L ;

Jarosch-Von Schweder, G ;

Haverich, A ;

Bader, A .

BIOTECHNOLOGY PROGRESS, 2000, 16 (01) :102-108

[7] Evaluation of cell behaviour related to physico-chemical properties of polymeric membranes to be used in bioartificial organs [J].

De Bartolo, L ;

Morelli, S ;

Bader, A ;

Drioli, E .

BIOMATERIALS, 2002, 23 (12) :2485-2497

[8] Galactose derivative immobilized glow discharge processed polyethersulfone membranes maintain the liver cell metabolic activity [J].

De Bartolo, Loredana ;

Morelli, Sabrina ;

Rende, Maria ;

Salerno, Simona ;

Giorno, Lidietta ;

Lopez, Linda C. ;

Favia, Pietro ;

d'Agostino, Riccardo ;

Drioli, Enrico .

JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (08) :2344-2353

[9] Stable plasma-deposited acrylic acid surfaces for cell culture applications [J].

Detomaso, L ;

Gristina, R ;

Senesi, GS ;

d'Agostino, R ;

Favia, P .

BIOMATERIALS, 2005, 26 (18) :3831-3841

[10] Plasma modified polymers as a support for enzyme immobilization 1. Allyl alcohol plasma [J].

Gancarz, I ;

Bryjak, J ;

Bryjak, M ;

Pozniak, G ;

Tylus, W .

EUROPEAN POLYMER JOURNAL, 2003, 39 (08) :1615-1622

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