Plasma modified polymers as a support for enzyme immobilization 1. Allyl alcohol plasma

被引:46
作者
Gancarz, I
Bryjak, J
Bryjak, M
Pozniak, G
Tylus, W
机构
[1] Wroclaw Univ Technol, Inst Organ & Polymer Technol, PL-50370 Wroclaw, Poland
[2] Wroclaw Univ Technol, Inst Chem Engn, PL-50370 Wroclaw, Poland
[3] Wroclaw Univ Technol, Inst Inorgan Technol, PL-50370 Wroclaw, Poland
关键词
plasma deposition; effect of argon; polysulfone; xylose isomerase;
D O I
10.1016/S0014-3057(03)00055-7
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
The paper describes deposition of plasma polymerized allyl alcohol on polysulfone film. It is shown that film surface becomes more hydrophilic after plasma treatment independently on presence of argon in a reaction mixture. The chemistry of the new surface layer was established by FTIR-ATR and ESCA spectroscopy. The substrate placed close to the plasma edge was the most hydrophilic but the amount of hydoxyl groups was not the highest there. Presence of argon stabilized the plasma but the deposited layer contained relatively less oxygen-bearing functionalities. The plasma treated polymer was subjected to xylose isomerase immobilization. For this purpose the divinylsulfone method was adapted. The studies revealed no correlation between the surface hydrophilicity and efficiency of immobilization. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1615 / 1622
页数:8
相关论文
共 63 条
[1]   THE FORMATION OF HIGH SURFACE CONCENTRATIONS OF HYDROXYL-GROUPS IN THE PLASMA POLYMERIZATION OF ALLYL ALCOHOL [J].
AMEEN, AP ;
SHORT, RD ;
WARD, RJ .
POLYMER, 1994, 35 (20) :4382-4391
[2]  
ANTRIM RL, 1979, APPL BIOCHEM BIOENG, V2, P98
[3]   Protein A immobilization and HIgG adsorption onto porous/nonporous and swellable HEMA-incorporated polyEGDMA microspheres [J].
Ayhan, H ;
Kesenci, K ;
Peskin, E .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2000, 11 (01) :13-25
[4]   Synthesis and characterization of heparinized polyurethanes using plasma glow discharge [J].
Bae, JS ;
Seo, EJ ;
Kang, IK .
BIOMATERIALS, 1999, 20 (06) :529-537
[5]   Plasma treatment of expanded PTFE offers a way to a biofunctionalization of its surface [J].
Baquey, C ;
Palumbo, F ;
Porte-Durrieu, MC ;
Legeay, G ;
Tressaud, A ;
d'Agostino, R .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1999, 151 (1-4) :255-262
[6]   Functionalization of nitrocellulose membranes using ammonia plasma for the covalent attachment of antibodies for use in membrane-based immunoassays [J].
Ben Rejeb, S ;
Tatoulian, M ;
Khonsari, FA ;
Durand, NF ;
Martel, A ;
Lawrence, JF ;
Amouroux, J ;
Le Goffic, F .
ANALYTICA CHIMICA ACTA, 1998, 376 (01) :133-138
[7]   Modification of polysulfone membranes 4. Ammonia plasma treatment [J].
Bryjak, M ;
Gancarz, I ;
Pozniak, G ;
Tylus, W .
EUROPEAN POLYMER JOURNAL, 2002, 38 (04) :717-726
[8]   Poly(EGDMA/AAm) copolymer beads: a novel carrier for enzyme immobilization [J].
Bulmus, V ;
Kesenci, K ;
Piskin, E .
REACTIVE & FUNCTIONAL POLYMERS, 1998, 38 (01) :1-9
[9]  
Chung TS, 1998, J APPL POLYM SCI, V68, P1677, DOI 10.1002/(SICI)1097-4628(19980606)68:10<1677::AID-APP17>3.0.CO
[10]  
2-U