Autophosphorylated calcium/calmodulin-dependent protein kinase II α induced by cerebral ischemia immediately targets and phosphorylates N-methyl-D-aspartate receptor subunit 2B (NR2B) in hippocampus of rats

被引：27

作者：

Meng, FJ ^{[1
]}

Zhang, GY ^{[1
]}

机构：

[1] Xuzhou Med Coll, Res Ctr Biochem & Mol Biol, Xuzhou 221002, Jiangsu, Peoples R China

来源：

NEUROSCIENCE LETTERS | 2002年 / 333卷 / 01期

基金：

中国国家自然科学基金;

关键词：

brain ischemia; calcium/calmodulin-dependent protein kinase II alpha; autophosphorylation; translocation; N-methyl-D-aspartate receptor; hippocampus; rat;

D O I：

10.1016/S0304-3940(02)00961-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

In this article, we investigated the autophosphorylation and translocation of calcium/calmodulin-dependent protein kinase II alpha (CaMKII alpha) in hippocampus during global ischemia. The following results were observed: (1) CaMKII a immediately became autophosphorylated after 3 min ischemia, at the same time, there is a dramatic and sustained translocation of CaMKII a from cytosolic fraction to synaptic fraction; (2) CaMKII a translocated to post-synaptic density and targeted N-methyl-D-aspartate receptor subunit 2B (NR2B) which was serine-phosphorylated by active CaMKII alpha; (3) serine phosphorylation of NR2B could not only inhibit the formation of CaMKII alpha-NR2B complexes but also promote the dissociation of the preformed complexes when ischemic time was prolonged. These results suggest that phosphorylation of NR2B can influence the channel properties of NR2B, and the dissociation of the CaMKII alpha-NR2B complexes may be a negative feedback mechanism during longer time cerebral ischemia. (C) 2002 Published by Elsevier Science Ireland Ltd.

引用

页码：59 / 63

页数：5