Identification of Respiratory Viruses in Adults: Nasopharyngeal versus Oropharyngeal Sampling

被引:109
作者
Lieberman, David [1 ,2 ]
Lieberman, Devora [2 ,3 ]
Shimoni, Avi [2 ]
Keren-Naus, Ayelet [4 ]
Steinberg, Rachel [4 ]
Shemer-Avni, Yonat [4 ]
机构
[1] Ben Gurion Univ Negev, Pulm Unit, Soroka Med Ctr, IL-84101 Beer Sheva, Israel
[2] Ben Gurion Univ Negev, Div Internal Med, Soroka Med Ctr, IL-84101 Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Dept Geriatr Med, Soroka Med Ctr, IL-84101 Beer Sheva, Israel
[4] Ben Gurion Univ Negev, Lab Clin Virol, Soroka Med Ctr, IL-84101 Beer Sheva, Israel
关键词
INFLUENZA-A; OPTICAL IMMUNOASSAY; MOLECULAR-DETECTION; FLOCKED SWABS; PCR; DIAGNOSIS; CHILDREN; INFECTIONS; COLLECTION; ASSAY;
D O I
10.1128/JCM.00886-09
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The optimal method for identifying respiratory viruses in adults has not been established. The objective of the study was to compare the sensitivities of three sampling methods for this purpose. One thousand participants (mean age, 63.1 +/- 17.8 years) were included. Of these, 550 were patients hospitalized for acute febrile lower respiratory tract infections and 450 were controls. Oropharyngeal swabs (OPS), nasopharyngeal swabs (NPS), and nasopharyngeal washings (NPW) were obtained from each participant and were tested for 12 respiratory viruses by a multiplex hydrolysis probes-based quantitative real-time reverse transcription-PCR. Patients were defined as positive for a specific virus if the virus was identified by at least one sampling method. In all, 251 viruses were identified in 244 participants. For the detection of any virus, the sensitivity rates for OPS, NPS, and NPW were 54.2%, 73.3%, and 84.9%, respectively (for OPS versus NPS and NPW, P < 0.00001; for NPS versus NPW, P < 0.003). Maximal sensitivity was obtained only with sampling by all three methods. The same gradation of sensitivity for the three sampling methods was found when influenza viruses, corona-viruses, and rhinoviruses were analyzed separately. The three sampling methods yielded equal sensitivity rates for respiratory syncytial virus. We conclude that nasopharyngeal sampling has a higher rate of sensitivity than oropharyngeal sampling and that the use of NPW has a higher rate of sensitivity than the use of NPS with a rigid cotton swab for the identification of respiratory viruses in adults. Sampling by all three methods is required for the maximal detection of respiratory viruses.
引用
收藏
页码:3439 / 3443
页数:5
相关论文
共 16 条
  • [11] Dry cotton or flocked respiratory swabs as a simple collection technique for the molecular detection of respiratory viruses using real-time NASBA
    Moore, Catherine
    Corden, Sally
    Sinha, Jaisi
    Jones, Rachel
    [J]. JOURNAL OF VIROLOGICAL METHODS, 2008, 153 (02) : 84 - 89
  • [12] Evaluation of an optical immunoassay for the rapid detection of influenza A and B viral antigens
    Schultze, D
    Thomas, Y
    Wunderli, W
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 2001, 20 (04) : 280 - 283
  • [13] Effectiveness of reverse transcription-PCR, virus isolation, and enzyme-linked immunosorbent assay for diagnosis of influenza a virus infection in different age groups
    Steininger, C
    Kundi, M
    Aberle, SW
    Aberle, JH
    Popow-Kraupp, T
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) : 2051 - 2056
  • [14] Comparative study of nasopharyngeal aspirate and nasal swab specimens for diagnosis of acute viral respiratory infection
    Sung, Rita Y. T.
    Chan, Paul K. S.
    Choi, Kai C.
    Yeung, Apple C. M.
    Li, Albert M.
    Tang, Julian W.
    Ip, Margaret
    Tsen, Tracy
    Nelson, E. Anthony S.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (09) : 3073 - 3076
  • [15] A sensitive, specific, and cost-effective multiplex reverse transcriptase-PCR assay for the detection of seven common respiratory viruses in respiratory samples
    Syrmis, MW
    Whiley, DM
    Thomas, M
    Mackay, IM
    Williamson, J
    Siebert, DJ
    Nissen, MD
    Sloots, TP
    [J]. JOURNAL OF MOLECULAR DIAGNOSTICS, 2004, 6 (02) : 125 - 131
  • [16] Comparison of respiratory virus detection rates for infants and toddlers by use of flocked swabs, saline aspirates, and saline aspirates mixed in universal transport medium for room temperature storage and shipping
    Walsh, Paul
    Overmyer, Christina Lim
    Pham, Kiemanh
    Michaelson, Scott
    Gofman, Larisa
    DeSalvia, Lisa
    Tran, Ty
    Gonzalez, Diana
    Pusavat, James
    Feola, Melanie
    Iacono, Kathryn T.
    Mordechai, Eli
    Adelson, Martin E.
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (07) : 2374 - 2376