Possible multistranded DNA induced by acid denaturation-renaturation

被引：1

作者：

Li, JW

Tian, F

Wang, C

Bai, CL

Cao, EH

机构：

[1] CHINESE ACAD SCI,INST CHEM,BEIJING 100080,PEOPLES R CHINA

[2] ACAD SINICA,INST BIOPHYS,BEIJING 100101,PEOPLES R CHINA

来源：

JOURNAL OF VACUUM SCIENCE & TECHNOLOGY B | 1997年 / 15卷 / 05期

关键词：

D O I：

10.1116/1.589563

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

lambda-DNA Hind III was denatured by HCl, renatured by NaOH, and completely digested by DNase I sequentially. The produced mixture was separated by gel filtration, and one component was obtained which showed resistance to the digestion of DNase 1. The results of atomic force microscopy (AFM) studies on this component indicated that an unusual form of DNA was presented, with the height 13 times that of double-stranded DNA. The renatured and partially digested lambda-DNA Hind III was also studied by AFM, and the images revealed both the duplex DNA and the unusual DNA. By comparison with known results, it is suggested that the unusual DNA is a multistranded DNA rather than a simple supercoil. (C) 1997 American Vacuum Society. [S0734-211X(97)01005-6].

引用

页码：1637 / 1640

页数：4

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